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Product Name HIRA Chinese Name 组蛋白替换精蛋白DGGR1抗体 Alias DGCR1; DGGR 1; DGGR1; DiGeorge critical region gene 1; HIR; HIR histone cell cycle regulation defective homolog A; HIRA; HIRA protein; HIRA_HUMAN; Protein HIRA; TUP 1; TUP1; TUP1 like enhancer of split protein 1; TUP1-like enhancer of split protein 1; TUPLE 1; TUPLE1. Research Area Cell biology immunology Developmental biology Chromatin and nuclear signals Stem cells Channel protein Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 112kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human HIRA/DGGR1: 251-350/1017 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq, Jul 2008]
Function:
Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
Subunit:
Interacts with histone H3F3B, PAX3 and PAX7 (By similarity). Interacts with CCNA1, HIRIP3, NFU1/HIRIP5 and histone H2B. Part of a complex which includes ASF1A, CABIN1, histone H3.3, histone H4 and UBN1.
Subcellular Location:
Nucleus. Nucleus, PML body. Note=Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence.
Tissue Specificity:
Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.
Post-translational modifications:
Sumoylated.
Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo.
Similarity:
Belongs to the WD repeat HIR1 family.
Contains 8 WD repeats.
SWISS:
P54198
Gene ID:
7290
Database links:Entrez Gene: 7290 Human
Entrez Gene: 15260 Mouse
Omim: 600237 Human
SwissProt: P54198 Human
SwissProt: Q61666 Mouse
Unigene: 474206 Human
Unigene: 15694 Mouse
Unigene: 453041 Mouse
Unigene: 14920 Rat
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