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Product Name WBSCR14 Chinese Name 糖类应答元件Binding proteinChREBP抗体 Alias ChREBP; bHLHd14; Carbohydrate responsive element binding protein; MIO; MLX interacting protein like; Mlx interactor; MONDOB; WBSCR 14; WBSCR14; MLXIPL;Williams Beuren syndrome chromosome region 14; Williams Beuren syndrome chromosome region 14 protein; WS basic helix loop helix leucine zipper protein; WS bHLH; MLXPL_HUMAN. Research Area Tumour Cardiovascular immunology Signal transduction transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Chicken, Dog, Pig, Cow, Horse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 93kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human WBSCR14/ChREBP: 81-180/852 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail ChREBP (Carbohydrate responsive element binding protein) is a transcription factor playing a critical role in the nutrient and hormonal regulation of genes encoding enzymes of glucose metabolism and lipogenesis pathways. It contains several domains including a nuclear localization signal (NLS) near the N-terminus, polyproline domains, a basic helix-loop-helix leucine zipper (b/HLH/Zip) and a leucine zipper like (zip-like) domain. ChREBP is ubiquitously detected in various tissues, with highest expression in liver, kidney and white and brown adipose tissue. Under basal conditions ChREBP is localized in the cytosol, translocating into the nucleus upon high glucose stimulation following its dephosphorylation of phospho-serine 196.
Function:
Transcriptional repressor. Binds to the canonical and non-canonical E box sequences 5'-CACGTG-3' (By similarity).
Subunit:
Binds DNA as a heterodimer with TCFL4/MLX.
Subcellular Location:
Nucleus.
Tissue Specificity:
Expressed in liver, heart, kidney, cerebellum and intestinal tissues.
Post-translational modifications:
Phosphorylation at Ser-556 by AMPK inactivates the DNA-binding activity (By similarity).
DISEASE:
Note=WBSCR14 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of WBSCR14 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
Similarity:
Contains 1 basic helix-loop-helix (bHLH) domain.
SWISS:
Q9NP71
Gene ID:
51085
Database links:Entrez Gene: 51085 Human
Omim: 605678 Human
SwissProt: Q9NP71 Human
Unigene: 647055 Human
ChREBP(又称碳水化合物反应元件Binding protein)是葡萄糖信号途径中的一个新的转录因子,在哺乳动物体内可结合到糖酵解和脂肪合成酶相关基因启动子区的糖类应答元件(ChRE)上, 在他们的共同作用下,可调节糖酵解和脂肪合成酶相关基因的表达.Product Picture Tissue/cell: rat rectum tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-WBSCR14 Polyclonal Antibody, Unconjugated(SL3516R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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