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Product Name CBX8 Chinese Name 染色质Binding proteinCbx8抗体 Alias Chromobox protein homolog 8; HPC3; Pc3; Polycomb 3 homolog; RC1; Rectachrome 1; CBX8_HUMAN. Research Area Chromatin and nuclear signals Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 43kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Cbx8: 51-150/389 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Cbx8, also known as Pc3, is a member of the Polycomb gene family and is required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. Cbx8 contains a chromodomain and is able to modify chromatin and histones.
Function:
Component of a Polycomb group (PcG) multiproteinPRC1-like complex, a complex class required to maintain thetranscriptionally repressive state of many genes, including Hoxgenes, throughout development. PcG PRC1 complex acts via chromatinremodeling and modification of histones; it mediatesmonoubiquitination of histone H2A 'Lys-119', rendering chromatinheritably changed in its expressibility.
Subunit:
Component of a PRC1-like complex. Interacts with RING1RNF2, PCGF1, PCGF2, PCGF3, BMI1, PCGF5 AND PCGF6. Interacts withMLLT3 and histone H3. Interacts with PHC2.
Subcellular Location:
Nucleus.
Similarity:
Contains 1 chromo domain.
SWISS:
Q9HC52
Gene ID:
57332
Database links:
Entrez Gene: 57332 Human
Entrez Gene: 30951 Mouse
SwissProt: Q9HC52 Human
SwissProt: Q9QXV1 Mouse
Unigene: 387258 Human
Unigene: 99953 Mouse
Unigene: 43722 Rat
Product Picture Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cbx8 Polyclonal Antibody, Unconjugated(SL3500R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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