Product Name	Phospho-p70 S6 Kinase Beta 2 (Tyr389)
Chinese Name	磷酸化核糖体S6蛋白激酶β2抗体
Alias	RPS6KB2(phospho Y389); S6K (phospho Y389); p-S6K(Tyr389); p70 S6 Kinase Beta 2 (phospho Tyr389); 70 kDa ribosomal protein S6 kinase 2; EC 2.7.11.1; KS6B2_HUMAN; p70 beta; p70 ribosomal S6 kinase beta; p70 S6 kinase beta; p70 S6K-beta; p70 S6KB; p70 S6Kbeta; p70(S6K) beta; p70-beta; p70-S6K 2; P70S6K2; p70S6Kb; Ribosomal protein S6 kinase 70kDa, polypeptide 2; Ribosomal protein S6 kinase beta 2; Ribosomal protein S6 kinase beta-2; Rps6kb2; S6 kinase related kinase; S6 kinase-related kinase; S6K beta 2; S6K beta; S6K-beta; S6K-beta-2; S6K2; Serine/threonine protein kinase 14 beta; Serine/threonine-protein kinase 14B; SRK; STK14B.
literatures	Specific References  (1)     |     SL3498R has been referenced in 1 publications. [IF=0.85] Hiyama et al. Nutrient starvation affects expression of LC3 family at the feto-maternal interface during murine placentation. (2015) J.Vet.Med.Sc. 77:305-11  WB ;  Mouse.   PubMed:25421500
Product Type	Phosphorylated anti
Research Area	Tumour  immunology  Signal transduction  Apoptosis  transcriptional regulatory factor  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Dog, Pig, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	53kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human RPS6KB2 around the phosphorylation site of Tyr389: FT(p-Y)VA
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Function: Phosphorylates specifically ribosomal protein S6. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. SWISS: Q9UBS0 Gene ID: 6199 Database links: Entrez Gene: 6199 Human Entrez Gene: 58988 Mouse Omim: 608939 Human SwissProt: Q9UBS0 Human SwissProt: Q9Z1M4 Mouse Unigene: 534345 Human Unigene: 271937 Mouse
Product Picture	Sample: Bone (Mouse) Lysate at 40 ug Primary: Anti- phospho-p70S6 Kinase Beta(Tyr389) (SL3498R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 53 kD Observed band size: 53 kD Sample: Brain(Rat) lysate at 30ug; Primary: Anti-Phospho-p70 S6 Kinase Beta 2(Tyr389) (SL3498R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size : 53kD Observed band size : 53kD Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (RPS6KB2) Polyclonal/MonoclonalAntibody, Unconjugated (SL3498R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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