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Product Name Phospho-TH (Ser31) Chinese Name 磷酸化酪氨酸羟化酶抗体 Alias Tyrosine Hydroxylase (Phospho-Ser31); Dystonia 14; DYT14; DYT5b; EC 1.14.16.2; OTTHUMP00000011225; OTTHUMP00000011226; ple; Protein Pale; TH; The; TY3H_RAT; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase. Product Type Phosphorylated anti Research Area Tumour Cell biology immunology Neurobiology Signal transduction Endocrinopathy Cell type markers The new supersedes the old Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 56kDa Cellular localization The nucleus cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from rat TH around the phosphorylation site of Ser31: VT(p-S)PR Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Tyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease.
Function:
Plays an important role in the physiology of adrenergic neurons.
Subunit:
Homotetramer.
Subcellular Location:
Mainly expressed in the brain and adrenal glands.
Post-translational modifications:
In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation.
Similarity:
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
SWISS:
P04177
Gene ID:
25085
Database links:Entrez Gene: 7054 Human
Entrez Gene: 21823 Mouse
Omim: 191290 Human
SwissProt: P07101 Human
SwissProt: P24529 Mouse
Unigene: 435609 Human
Unigene: 1292 Mouse
Unigene: 11082 Rat
神经细胞Maker
酪氨酸羟化酶(TH)是儿茶酚胺类神经递质即多巴胺、去甲肾上腺素、肾上腺素生物合成过程所需的限速酶,它以四氢生物喋呤啶(BH4)为辅酶,催化酪氨酸的羟化而生成多巴(DOPA)。
已知在患帕金森病(Parkinson disease,PD)时,脑内多巴胺(dopamine,DA)的减少与此酶活性低下有关。因此对PD模型动物来说,若将TH基因植入脑内,便可以提高脑内DA水平而达到基因治疗目的。Product Picture Sample:
Lane 1: Adrenal gland (Mouse) Lysate at 40 ug
Lane 2: Adrenal gland (Rat) Lysate at 40 ug
Primary: Anti-Phospho-TH (Ser31) (SL3463R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 59 kD
Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (SL3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (SL3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (SL3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-TH (Ser31)) Polyclonal Antibody, Unconjugated (SL3463R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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