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Product Name Phospho-Stat2 (Tyr690) Chinese Name 磷酸化Signal transduction和转录激活因子2抗体 Alias Stat2 (phospho-Tyr690); Stat2 (phospho Y690); Stat2 (phospho Tyr690); p-Stat2 (Tyr690); signal transducers and activators of transduction2; Homo sapiens interferon alpha induced transcriptional activator; interferon alpha induced transcriptional activator; ISGF3; P113; signal transducer and activator of transcription 2 113kD; STAT113; STAT2_HUMAN; p113. literatures Specific References (4) | SL3428R has been referenced in 4 publications.[IF=6.448] Collotta D et al. Baricitinib counteracts metaflammation, thus protecting against diet-induced metabolic abnormalities in mice. Mol Metab .2020 Sep;39:101009. WB ; Mouse.[IF=6.304] Chen Y et al. Dendritic cells-derived interferon-λ1 ameliorated inflammatory bone destruction through inhibiting osteoclastogenesis. Cell Death Dis. 2020 Jun 2;11(6):414. WB ; Mouse.[IF=6.291] Changjiang Liu. et al. Cypermethrin triggers YY1-mediated testosterone biosynthesis suppression. Ecotox Environ Safe. 2021 Dec;225:112792 WB ; Rat.[IF=5.162] Zhuo Han. et al. New insights into Vitamin C function: Vitamin C induces JAK2 activation through its receptor-like transporter SVCT2. Int J Biol Macromol. 2021 Mar;173:379 WB ; Human.Product Type Phosphorylated anti Research Area Tumour Cell biology immunology Apoptosis transcriptional regulatory factor Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 94kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human Stat2 around the phosphorylation site of Tyr690: RK(p-Y)LK Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010].
Function:
Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Subunit:
Interacts with ISGF3G/IRF-9 in the cytoplasm. Heterodimer with STAT1 upon IFN-alpha/beta induced phosphorylation. Interacts with CRSP2 and CRSP6. Interacts with Simian virus 5 protein V and rabies virus phosphoprotein (By similarity). Can form a homodimer upon IFN-alpha induced phosphorylation. Interacts with IFNAR1; the interaction requires the phosphorylation of IFNAR1 at 'Tyr-466'. Interacts with IFNAR2. Interacts with dengue virus NS5; this interaction inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), targets STAT2 for degradation. Interacts with human cytomegalovirus/HHV-5 protein UL123; this interaction promotes viral growth.
Subcellular Location:
Cytoplasm. Nucleus. Note=Translocated into the nucleus upon activation by IFN-alpha/beta.
Post-translational modifications:
Tyrosine phosphorylated in response to IFN-alpha.
Similarity:
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
SWISS:
P52630
Gene ID:
6773
Database links:Entrez Gene: 6773 Human
Entrez Gene: 20847 Mouse
Omim: 600556 Human
SwissProt: P52630 Human
SwissProt: Q9WVL2 Mouse
Unigene: 530595 Human
Unigene: 293120 Mouse
Unigene: 471333 Mouse
Unigene: 24237 Rat
STAT-Signal transduction和转录激活因子蛋白家族是近年来发现的一类转录因子.通过JAK-STAT途径介导许多由cell factor诱导的反应,参与细胞的Signal transduction和转录。Product Picture Sample:Brain (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-Stat2 (Tyr690)(SL3428R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 94kD
Observed band size: 105kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Stat2 (Tyr690)) Polyclonal Antibody, Unconjugated (SL3428R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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