Product Name	Phospho-TORC2 (Ser171)
Chinese Name	磷酸化CREB转录共激活因子TORC2抗体
Alias	TORC2 (phospho S171); TORC2 (phospho Ser171); p-TORC2 (Ser171); CREB regulated transcription coactivator 2; CRTC2; RP11-422P24.6; TORC-2; Transducer of CREB protein 2; Transducer of regulated cAMP response element-binding protein; Transducer of regulated cAMP response element-binding protein (CREB) 2; Transducer of regulated cAMP response element-binding protein 2; Transducer of regulated CREB protein 2; CRTC2_HUMAN.
literatures	Specific References  (5)     |     SL3415R has been referenced in 5 publications. [IF=40.73] Seok S et al. Transcriptional regulation of autophagy by an FXR-CREB axis. (2014) Natur. 516:108-11  WB ;  Mouse.   PubMed:25383523 [IF=5.5] Cicerchi et al. Uric acid-dependent inhibition of AMP kinase induces hepatic glucose production in diabetes and starvation: evolutionary implications of the uricase loss in hominids. (2014) FASEB.. 28:3339-50  WB ;  Human.   PubMed:24755741 [IF=3.562] Zhou TT et al. Small molecule IVQ, as a prodrug of gluconeogenesis inhibitor QVO, efficiently ameliorates glucose homeostasis in type 2 diabetic mice.Acta Pharmacol Sin. 2019 Mar 4.  WB ;  Mouse.   PubMed:30833709 [IF=3.02] Slocum, Stephen L., et al. "Keap1/Nrf2 pathway activation leads to a repressed hepatic gluconeogenic and lipogenic program in mice on a high-fat diet." Archives of Biochemistry and Biophysics (2016).  WB ;  Mouse.   PubMed:26701603 [IF=2.09] Tian, Xiaohui, et al. "CRTC2 enhances HBV transcription and replication by inducing PGC1alpha expression." Virology Journal 11.1 (2014): 30.  WB ;  Human.   PubMed:24529027
Product Type	Phosphorylated anti
Research Area	Tumour  immunology  Signal transduction  Apoptosis  transcriptional regulatory factor  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1ug/Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	73kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human CREB regulated transcription coactivator 2 around the phosphorylation site of Ser171: TS(p-S)DS
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2010]. Function: Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). Subunit: Binds, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1. 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction. Interaction, via its C-terminal, with TAF4, enhances recruitment of TAF4 to CREB1. Interacts with PPP3CA/calcineurin alpha, SIK2 and 14-3-3 proteins, YWHAB and YWHAG. Interaction with the human T-cell leukemia virus type 1 (HTLV-1) Tax protein is essential for optimal transcription activation by Tax. Interaction with RFWD2/COP1 mediates nuclear export and degradation of CRTC2. Subcellular Location: Cytoplasm. Nucleus. Note=Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus. Tissue Specificity: Most abundantly expressed in the thymus. Present in both B and T-lymphocytes. Highly expressed in HEK293T cells and in insulinomas. High levels also in spleen, ovary, muscle and lung, with highest levels in muscle. Lower levels found in brain, colon, heart, kidney, prostate, small intestine and stomach. Weak expression in liver and pancreas. Post-translational modifications: Phosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs (SIK1 and SIK2) by LKB1. Both insulin and AMPK increase this phosphorylation of CRTC2 while glucagon suppresses it. Phosphorylation at Ser-274 by MARK2 is induced under low glucose conditions and dephosphorylated in response to glucose influx. Phosphorylation at Ser-274 promotes interaction with 14-3-3 proteins and translocation to the cytoplasm. Similarity: Belongs to the TORC family. SWISS: Q53ET0 Gene ID: 200186 Database links: Entrez Gene: 200186 Human Entrez Gene: 74343 Mouse Entrez Gene: 310615 Rat Omim: 608972 Human SwissProt: Q53ET0 Human SwissProt: Q3U182 Mouse SwissProt: Q3LRZ1 Rat Unigene: 406392 Human Unigene: 35627 Mouse Unigene: 13599 Rat CREB转录共激活因子TORC2又称葡萄糖关键调控蛋白TORC-2。
Product Picture	Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug Lane 2: Spleen (Mouse) Lysate at 40 ug Primary: Anti-Phospho-TORC2 (Ser171) (SL3415R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 95 kD Blank control: A431. Primary Antibody (green line): Rabbit Anti-Phospho-TORC2 (Ser171) antibody (SL3415R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control: A431. Primary Antibody (green line): Rabbit Anti-Phospho-TORC2 (Ser171) antibody (SL3415R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control: Jurkat. Primary Antibody (green line): Rabbit Anti-Phospho-TORC2 (Ser171) antibody (SL3415R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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