Product Name	Phospho-RPS6 (Ser240 + Ser244)
Chinese Name	磷酸化S6核糖体蛋白(Ser240/244)抗体
Alias	RPS6 (phospho S240 + S244); p-RPS6 (phospho S240 + S244); Air8; DS6; Hemocytes enlarged; Hen; L(1)AIR8; Lethal (1) haemocytes enlarged; Lethal aberrant immune response 8; Lethal(1)abberant immune response[8]; Phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S6; RPS 6; RpS6; RS6; RS6_HUMAN; S6; S6 Ribosomal Protein.
Product Type	Phosphorylated anti
Research Area	Chromatin and nuclear signals  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:100-1000  ELISA=1:100-1000  IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	29kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human RPS6 around the phosphorylation site of Ser240/244: RA(p-S)TSK(p-S)ES
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008] Function: May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Post-translational modifications: Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphrylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex. Similarity: Belongs to the ribosomal protein S6e family. SWISS: P62753 Gene ID: 6194 Database links: Entrez Gene: 6194 Human Entrez Gene: 20104 Mouse Entrez Gene: 667739 Mouse Entrez Gene: 29304 Rat Omim: 180460 Human SwissProt: P62753 Human SwissProt: P62754 Mouse SwissProt: P62755 Rat Unigene: 408073 Human Unigene: 379007 Mouse Unigene: 425090 Mouse Unigene: 34341 Rat
Product Picture	Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti-Phospho-RPS6 (Ser240 + Ser244) (SL3389R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-Phospho-RPS6 (Ser240 + Ser244) (SL3389R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 29 kD Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with(Phospho-RPS6(Ser240+Ser244)) Polyclonal Antibody, Unconjugated (SL3389R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-RPS6 (Ser240+Ser244) Polyclonal Antibody, Unconjugated(SL3389R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-RPS6 (Ser240+Ser244) Polyclonal Antibody, Unconjugated(SL3389R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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