Product Name	phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199)
Chinese Name	磷酸化磷脂酰肌醇激酶/PI3 Kinase P85α/γ抗体
Alias	PI3 kinase p85 alpha (phospho Tyr467); PI3 kinase p55 gamma (phospho Tyr199); phospho-PI3 kinase p85 alpha / gamma (Tyr467 / Tyr199); PI3-kinase p85 subunit alpha; PI3-kinase p85 subunit gamma; P85A_HUMAN; Phosphatidylinositol 3-kinase regulatory subunit alpha; GRB1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha (PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha); PI 3-kinase p85α; PI 3-kinase p85 α; PI 3-kinase p85-α; SH3_PI3K_p85alpha; PI3-kinase p85 subunit alpha; phosphoinositide-3-kinase regulatory subunit 1; p85; AGM7; IMD36; p85-ALPHA; PI 3 Kinase p85 alpha;
literatures	Specific References  (26)     |     SL3332R has been referenced in 26 publications. [IF=6.208] Wen-Ling Liao. et al. Inhibitory Effects of Ursolic Acid on the Stemness and Progression of Human Breast Cancer Cells by Modulating Argonaute-2. INT J MOL SCI. 2023 Jan;24(1):366  WB ;  Human.   PubMed:36613808 [IF=5.742] Feng Liu. et al. CircRNA_0084927 promotes colorectal cancer progression by regulating miRNA-20b-3p/glutathione S-transferase mu 5 axis. World J Gastroentero. 2021 Sep 28; 27(36): 6064–6078  WB ;  Human.   PubMed:34629820 [IF=5.614] Zhu J et al. SPARC Promotes Self‐Renewal of Limbal Epithelial Stem Cells and Ocular Surface Restoration through JNK and p38‐MAPK Signaling Pathways. Stem Cells. 2019 Oct 23.  WB ;  Rabbit.   PubMed:31644832 [IF=5.455] Ma, Zhaochen. et al. Repurposing a clinically approved prescription Colquhounia root tablet to treat diabetic kidney disease via suppressing PI3K/AKT/NF-kB activation. Chin Med-Uk. 2022 Dec;17(1):1-13  WB ;  Rat.   PubMed:34980163 [IF=4.546] Huang, Wenjie. et al. The inhibitory effect and mechanism of Yi-qi-hua-yu-jie-du decoction on the drug resistance of gastric cancer stem cells based on ABC transporters. CHIN MED-UK. 2022 Dec;17(1):1-18  WB ;  Human.   PubMed:35941687 [IF=4.014] Hanpeng Wu. et al. The release of FB1-induced heterophil extracellular traps in chicken is dependent on autophagy and glycolysis. POULTRY SCIENCE. 2023 Jan;:102511  WB ;  Chicken.   PubMed:10.1016/j.psj.2023.102511 [IF=4.011] Zhou C et al. Combining transcatheter arterial embolization with iodized oil containing Apatinib inhibits HCC growth and metastasis. Sci Rep. 2020 Feb 19;10(1):2964.  WB&ICF ;  Human.   PubMed:32076049 [IF=3.943] Di Wu. et al. Alpinetin prevents inflammatory responses in OVA-induced allergic asthma through modulating PI3K/AKT/NF-κB and HO-1 signaling pathways in mice. Int Immunopharmacol. 2020 Dec;89:107073  WB ;  Mouse.   PubMed:33039967 [IF=3.932] Li Ling. et al. A novel peptide promotes human trophoblast proliferation and migration through PI3K/Akt/mTOR signaling pathway. Ann Transl Med. 2021 Jun; 9(12): 981  WB ;  Human.   PubMed:34277781 [IF=3.932] Jing Chen. et al. Apatinib enhances the anti-tumor effect of paclitaxel via the PI3K/p65/Bcl-xl pathway in triple-negative breast cancer. Ann Transl Med. 2021 Jun; 9(12): 1001  WB ;  Human.   PubMed:34277801 [IF=3.895] Yin H et al. T-2 Toxin Induces Oxidative Stress, Apoptosis and Cytoprotective Autophagy in Chicken Hepatocytes. Toxins (Basel). 2020 Jan 29;12(2).  WB ;  Chicken.   PubMed:32013230 [IF=3.887] Yan LI. et al. The combination of EGCG with warfarin reduces deep vein thrombosis in rabbits through modulating HIF-1α and VEGF via the PI3K/AKT and ERK1/2 signaling pathways. CHIN J NAT MEDICINES. 2022 Sep;20:679  WB ;  Human.   PubMed:10.1016/S1875-5364(22)60172-9 [IF=3.73] Padiya, Raju, et al. "Garlic Attenuates Cardiac Oxidative Stress via Activation of PI3K/AKT/Nrf2-Keap1 Pathway in Fructose-Fed Diabetic Rat." PLOS ONE 9.5 (2014): e94228.  WB ;  Rat.   PubMed:24796753 [IF=3.361] Jiang A et al. Morin alleviates LPS-induced mastitis by inhibiting the PI3K/AKT, MAPK, NF-κB and NLRP3 signaling pathway and protecting the integrity of blood-milk barrier. Int Immunopharmacol. 2019 Nov 8:105972.  WB ;  Mouse.   PubMed:31711938 [IF=3.35] Jeong, Heesoo, Yanan Liu, and Hyun-Sook Kim. "Dried plum and chokeberry ameliorate d-galactose-induced aging in mice by regulation of Pl3k/Akt-mediated Nrf2 and Nf-kB pathways." Experimental Gerontology (2017).  WB ;  Mouse.   PubMed:28502776 [IF=3.266] Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.  WB ;  Mouse.   PubMed:31497217 [IF=3.17] Yang RP et al. Pharmacological actions of neferine in the modulation of human platelet function. Eur J Pharmacol. 2019 Aug 21:172626.  WB ;  Human.   PubMed:31445013 [IF=3.098] Lu Xue. et al. A novel peptide relieves endothelial cell dysfunction in preeclampsia by regulating the PI3K/mTOR/HIF1α pathway. Int J Mol Med. 2021 Jan;47(1):276-288  WB ;  Human.   PubMed:33236147 [IF=3.046] Qiao J et al. Knockdown of ROS proto-oncogene 1 inhibits migration and invasion in gastric cancer cells by targeting the PI3K/Akt signaling pathway. OncoTargets and Therapy, 2019,12: 8569–8582.  WB ;  Human.   PubMed:doi:10.2147/ott.s213421 [IF=2.394] Liu W et al. Synergistic effect of tolfenamic acid and glycyrrhizic acid on TPA-induced skin inflammation in mice. MedChemComm.2019.  IHC-P ;  Mouse.   PubMed:doi:10.1039/c9md00345b [IF=2.342] Liu W et al. Glycyrrhizic acid from licorice down-regulates inflammatory responses via blocking MAPK and PI3K/Akt-dependent NF-κB signalling pathways in TPA-induced skin inflammation. Medchemcomm. 2018 Jul 19;9(9):1502-1510.  IHC-P ;  Mouse.   PubMed:30288224 [IF=2.342] Liu W et al. Glycyrrhizic acid from licorice down-regulates inflammatory responses via blocking MAPK and PI3K/Akt-dependent NF-κB signalling pathways in TPA-induced skin inflammation. MedChemComm. 2018.  IHC-P ;  Mouse.   PubMed:doi:10.1039/c8md00288f [IF=1.851] Zhang X et al. COX-2 promotes epithelial‑mesenchymal transition and migration in osteosarcoma MG‑63 cells via PI3K/AKT/NF‑κB signaling. Molecular Medicine REPORTS.2019 20: 3811-3819.  WB ;  Human.   PubMed:doi:10.3892/mmr.2019.10598 [IF=1.84] Sun, Yan, et al. "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." Theriogenology (2014).  WB ;   PubMed:25284282 [IF=1.813] Fan Q et al. Phenylethanol Glycosides Protect Myocardial Hypertrophy Induced by Abdominal Aortic Constriction via ECE-1 Demethylation Inhibition and PI3K/PKB/eNOS Pathway Enhancement. Evid Based Complement Alternat Med. 2020 Jun 9;2020:2957094.  WB ;  Rat.   PubMed:32595726 [IF=1.785] Zhongbai Zhang. et al. Oxymatrine pretreatment protects H9c2 cardiomyocytes from hypoxia/reoxygenation injury by modulating the PI3K/Akt pathway. Exp Ther Med. 2021 Jun;21(6):1-12  WB ;  Rat.   PubMed:33850528
Product Type	Phosphorylated anti
Research Area	Tumour  immunology  Signal transduction  Apoptosis  transcriptional regulatory factor  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	85/55kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human PI3 kinase p85 alpha around the phosphorylation site of Tyr467: RL(p-Y)EE
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Function: Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Subunit: Heterodimer of a regulatory subunit PIK3R1 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with FER. Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated). Interacts with PTK2/FAK1. Interacts with phosphorylated TOM1L1. Interacts with phosphorylated LIME1 upon TCR and/or BCR activation. Interacts with SOCS7. Interacts with RUFY3. Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated). Interacts with LYN (via SH3 domain); this enhances enzyme activity. Interacts with phosphorylated LAT, LAX1 and TRAT1 upon TCR activation. Interacts with CBLB. Interacts with HIV-1 Nef to activate the Nef associated p21-activated kinase (PAK). This interaction depends on the C-terminus of both proteins and leads to increased production of HIV. Interacts with HCV NS5A. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with IRS1 and phosphorylated IRS4, as well as with NISCH and HCST. Interacts with FASLG, KIT and BCR. Interacts with AXL, FGFR1, FGFR2, FGFR3 and FGFR4 (phosphorylated). Interacts with FGR and HCK. Interacts with PDGFRA (tyrosine phosphorylated) and PDGFRB (tyrosine phosphorylated). Interacts with ERBB4 (phosphorylated). Interacts with NTRK1 (phosphorylated upon ligand-binding). Subcellular Location: Cytoplasmic. Tissue Specificity: Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level). Post-translational modifications: Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation. Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear. Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR. Similarity: Belongs to the PI3K p85 subunit family. Contains 1 Rho-GAP domain. Contains 2 SH2 domains. Contains 1 SH3 domain. SWISS: P27986 Gene ID: 5295 Database links: Entrez Gene: 5295 Human Entrez Gene: 8503 Human Entrez Gene: 18708 Mouse Entrez Gene: 18710 Mouse Entrez Gene: 25513 Rat Entrez Gene: 60664 Rat Omim: 171833 Human Omim: 606076 Human SwissProt: P27986 Human SwissProt: Q92569 Human SwissProt: P26450 Mouse SwissProt: Q64143 Mouse SwissProt: Q63787 Rat SwissProt: Q63789 Rat Unigene: 132225 Human Unigene: 170510 Human Unigene: 253819 Mouse Unigene: 44448 Rat
Product Picture	Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-PI3 kinase p85 alpha+gamma (Tyr467+Tyr199) Polyclonal Antibody, Unconjugated(SL3332R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: Molt4. Primary Antibody (green line): Rabbit Anti- phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199)/FITC Conjugated antibody (SL3332R-FITC) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG-FITC . Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

Cartpieces

• 
• 1

  1Delete

• 
• 5

  5Delete

• 
• S

  SDelete

• 
• R

  RDelete

• 
• +

  +Delete

• 
• 2

  2Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• P

  PDelete

• 
• P

  PDelete

• 

• Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• R

  RDelete

• 
• 5

  5Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout