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Product Name Phospho-PBK (Thr9) Chinese Name 磷酸化PDZ连接激酶/T-LAK细胞源蛋白激酶抗体 Alias PBK (phospho T9); PBK (phospho Thr9); Cancer/testis antigen 84; CT84; FLJ14385; Lymphokine activated killer T cell originated protein kinase; MAPKK like protein kinase; PDZ-binding-kinase/T-LAK cell-originated protein kinase; Nori 3; Nori3; PDZ binding kinase; Serine/threonine protein kinase; Spermatogenesis related protein kinase; SPK; T LAK cell originated protein kinase; TOPK. Product Type Phosphorylated anti Research Area Tumour immunology Signal transduction Apoptosis transcriptional regulatory factor Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 36kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human PBK/TOPK around the phosphorylation site of Thr9: FK(p-T)P Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Function:
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Subunit:
Interacts with DLG1 and TP53.
Tissue Specificity:
Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
Post-translational modifications:
Phosphorylated; in a cell-cycle dependent manner at mitosis.
Similarity:
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
SWISS:
Q96KB5
Gene ID:
55872
Database links:Entrez Gene: 55872 Human
Omim: 611210 Human
SwissProt: Q96KB5 Human
Unigene: 104741 Human
PBK/TOPK是一种新近发现的丝-苏氨酸激酶,属于MAPKK分子家族成员,具有参与调控恶性Tumour细胞的增殖和周期变化, 促进Tumour细胞转化, 并且通过MAPKK信号通路参与调控细胞DNA损伤修复.Product Picture
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