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Product Name S-tag Chinese Name S-tagTag anti Alias Stag; S tag; Ribonuclease A; KETAAAKFERQHMDS. literatures Specific References (1) | SL33017R has been referenced in 1 publications.[IF=5.486] Zhangji Dong. et al. Histone acetyltransferase KAT2A modulates neural stem cell differentiation and proliferation by inducing degradation of the transcription factor PAX6. J BIOL CHEM. 2023 Feb;:103020 IP ; Human.Product Type Tag anti Research Area Cell biology Immunogen Species Rabbit Clonality Polyclonal React Species Recombinant protein Applications WB=1:1000-5000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide S-tag Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). If RNase A is digested with subtilisin, a single peptide bond is cleaved, but the resulting two products remain weakly bound to each other and the protein, called ribonuclease S, remains active although each of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag.
The amino acid sequence of the S-tag is: Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser. It is believed that the peptide with its abundance of charged and polar residues could improve solubility of proteins it is attached to[citation needed]. Moreover, the peptide alone is thought not to fold into a distinct structure. On DNA-level the S-tag can be attached to the N- or C-terminus of any protein. After gene expression, such a tagged protein can be detected by commercially available antibodies.
SWISS:
N/A
Gene ID:
N/A
Product Picture Sample:
Lane1: S-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug
Lane2: S-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug
Primary: Anti-S-tag (SL33017R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 45 kD
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