Product Name	Phospho-MYL (Ser19)
Chinese Name	磷酸化心脏肌球蛋白轻链2抗体
Alias	Cardiac myosin light chain-2; Cardiac ventricular myosin light chain 2; CMH10; MLC 2v; MLC2; MYL 2; Myosin light chain 2 regulatory cardiac slow; Myosin light polypeptide 2 regulatory cardiac slow; Myosin regulatory light chain 2 ventricular cardiac muscle isoform; Myosin regulatory light chain 2; ventricular/cardiac muscle isoform; Regulatory light chain of myosin; RLC of myosin; Slow cardiac myosin regulatory light chain 2; MLRV_HUMAN.
Product Type	Phosphorylated anti
Research Area	Cardiovascular  Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	18kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human MYL2 around the phosphorylation site of Ser19: VF(p-S)MF
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008] Subunit: Myosin is a hexamer of 2 heavy chains and 4 light chains. Subcellular Location: Cytoplasm, myofibril, sarcomere, A band Post-translational modifications: N-terminus is methylated by METTL11A/NTM1. Phosphorylated by MYLK3. DISEASE: Defects in MYL2 are the cause of familial hypertrophic cardiomyopathy type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Similarity: Contains 3 EF-hand domains. SWISS: P10916 Gene ID: 4633 Database links: Entrez Gene: 4633 Human Entrez Gene: 17906 Mouse Entrez Gene: 363925 Rat Omim: 160781 Human SwissProt: P10916 Human SwissProt: P51667 Mouse SwissProt: P08733 Rat Unigene: 75535 Human Unigene: 1529 Mouse Unigene: 37176 Rat Unigene: 6534 Rat
Product Picture	Sample: Heart (Rat) Lysate at 40 ug Primary: Anti-Phospho-MYL(Ser19) (SL3295R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD Tissue/cell: smooth muscle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-MYL(Ser19) Polyclonal Antibody, Unconjugated(SL3295R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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