Product Name	Phospho-MYPT1 (Thr696)
Chinese Name	磷酸化肌球蛋白磷酸酶抗体
Alias	MYPT1 (Phospho Thr696); MYPT1 (Phospho T696); Myosin Phosphatase (phospho T696); Myosin Phosphatase (phospho Thr696); p-MYPT1(Thr696); Myosin Phosphatase; M130; MBS; MGC133042; Myosin phosphatase target subunit 1; Myosin phosphatase targeting subunit 1; MYPT 1; MYPT1; PPP1R12A; Protein phosphatase 1 regulatory inhibitor subunit 12A; Protein phosphatase 1 regulatory subunit 12A; Protein phosphatase myosin binding subunit; MYPT1_HUMAN; Myosin phosphatase-targeting subunit 1; Protein phosphatase myosin-binding subunit.
literatures	Specific References  (3)     |     SL3287R has been referenced in 3 publications. [IF=5.396] Luqing Song. et al. Chlorogenic acid improves the intestinal barrier by relieving endoplasmic reticulum stress and inhibiting ROCK/MLCK signaling pathways.. Food Funct. 2022 Feb;:  WB ;  Human.   PubMed:10.1039/D1FO02662C [IF=4.254] Kwon Y et al. Involvement of inhibitor kappa B kinase 2 (IKK2) in the regulation of vascular tone.Lab Invest. 2018 Oct;98(10):1311-1319.  IF ;  Rat.   PubMed:29785049 [IF=0.948] Guiyuan Liu. et al. Rhubarb Anthraquinones Ameliorates Inflammatory Lung Injury by Enhancing Alveolar Epithelium Tight Junction Proteins through RhoA/ROCK1 Signalling. PHARMACOGN MAG. ;():  WB ;  Mouse.   PubMed:10.1177/09731296231158697
Product Type	Phosphorylated anti
Research Area	Tumour  immunology  Signal transduction  Apoptosis  transcriptional regulatory factor  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 Flow-Cyt=1μg /Test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	113kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human MYPT1 around the phosphorylation site of Thr696: RS(p-T)QG
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in non muscle cells. The guanosine triphosphate (GTP) bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho associated kinase (Rho kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Therefore Rho appears to inhibit myosin phosphatase through the action of Rho kinase. Function: Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity. Subunit: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT. Binds PPP1R12B, ROCK1 and IL16. Interacts directly with PRKG1. Non-covalent dimer of 2 dimers; PRKG1-PRKG1 and PPP1R12A-PPP1R12A. Interacts with SMTNL1. Interacts with PPP1CB; the interaction is direct. Interacts (when phosphorylated at Ser-445, Ser-472 and Ser-910) with 14-3-3. Interacts with ROCK1 and ROCK2. Interacts with isoform 1 and isoform 2 of ZIPK/DAPK3. Interacts with RAF1. Interacts with HIF1AN. Subcellular Location: Cytoplasm. Note=Along actomyosin filaments and stress fibers. Tissue Specificity: Expressed in striated muscles, specifically in type 2a fibers (at protein level). Post-translational modifications: Phosphorylated by CIT (Rho-associated kinase). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. Phosphorylation at Ser-445, Ser-472 and Ser-910 by NUAK1 promotes interaction with 14-3-3, leading to inhibit interaction with myosin light chain MLC2, preventing dephosphorylation of MLC2. May be phosphorylated at Thr-696 by DMPK; may inhibit the myosin phosphatase activity. Phosphorylated at Ser-473 by CDK1 during mitosis, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates PPP1R12A. Similarity: Contains 6 ANK repeats. SWISS: O14974 Gene ID: 4659 Database links: Entrez Gene: 4659 Human Entrez Gene: 17931 Mouse Entrez Gene: 116670 Rat Omim: 602021 Human SwissProt: O14974 Human SwissProt: Q9DBR7 Mouse SwissProt: Q10728 Rat Unigene: 49582 Human Unigene: 422959 Mouse Unigene: 482714 Mouse Unigene: 162937 Rat
Product Picture	Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Human HeLa cell lysates Lane 3: Human MCF-7 cell lysates Primary: Anti-Phospho-MYPT1 (Thr696) (SL3287R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 113 kDa Observed band size: 125 kDa Sample: BRL-3A (Rat)cell Lysate at 40 ug Primary: Anti-p-MYPT1(Thr696) (SL3287R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 113kD Observed band size: 120 kD Sample: 293T (human)cell Lysate at 40 ug Primary: Anti-p-MYPT1(Thr696) (SL3287R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 113kD Observed band size: 120 kD Blank control(blue): Hela cells (fixed with 2% paraformaldehyde (10 min),then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti-Phospho-MYPT1(Thr696) antibody(SL3287R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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