Product Name	Phospho-MARCKS (Ser152 + Ser156)
Chinese Name	磷酸化丙氨酸蛋白激酶C底物Marcks抗体
Alias	MARCKS (phospho S152 + S156); p-MARCKS (phospho S152 + S156); MARCKS (phospho S159 + S163); 80 kDa protein; 80K L; 80K L protein; 80K-L protein; 80KL; 81 kDa protein, light chain; light chain; MACS; MARCKS; MARCS; MARCS_RAT; MGC52672; myristoylated alanine rich C kinase substrate; Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L); Myristoylated alanine rich protein kinase C substrate; Myristoylated alanine-rich C-kinase substrate; Phosphomyristin; PKCSL; PRKCSL; protein kinase C substrate 80 kDa protein light chain; Protein kinase C substrate.
Product Type	Phosphorylated anti
Research Area	Cardiovascular  immunology  Signal transduction  Kinases and Phosphatases  Cytoskeleton
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Chicken, Dog, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	30kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from rat MARCKS around the phosphorylation site of Ser152/156 or Ser159/163(human) : RF(p-S)FKK(p-S)FK
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008] Function: MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. Subcellular Location: Cytoplasm, cytoskeleton (Probable). Membrane; Lipid-anchor. Tissue Specificity: Highest levels found in spleen and brain. Intermediate levels seen in thymus, ovary, lung and heart. Very low levels seen in kidney, skeletal muscle and liver. Post-translational modifications: Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. Similarity: Belongs to the MARCKS family. SWISS: P29966 Gene ID: 25603 Database links: Entrez Gene: 4082 Human Entrez Gene: 17118 Mouse Entrez Gene: 25603 Rat Omim: 177061 Human SwissProt: P29966 Human SwissProt: P26645 Mouse SwissProt: P30009 Rat Unigene: 519909 Human Unigene: 712721 Human Unigene: 30059 Mouse Unigene: 9560 Rat MARCKS是一种分子量为32kDa的膜内蛋白,在组织中广泛表达,但主要高表达在脑内的轴突、小直径的树突和树突棘.
Product Picture	Sample: Spleen (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Primary: Anti- Phospho-MARCKS (Ser152 + Ser156) (SL3263R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD Paraformaldehyde-fixed, paraffin embedded (Rat Cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-MARCKS (Ser152 + Ser156)) Polyclonal Antibody, Unconjugated (SL3263R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-MARCKS (Ser152 + Ser156)) Polyclonal Antibody, Unconjugated (SL3263R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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