Product Name	Phospho-ALOX5 (Ser663)
Chinese Name	磷酸化5-脂氧合酶抗体
Alias	5-Lipoxygenase (Phospho Ser663); 5-Lipoxygenase (Phospho S663); 5-lipoxygenase; 5 LO; 5LPG; ALOX 5; ALOX5; Arachidonate 5 lipoxygenase; Arachidonic acid 5 lipoxygenase; Leukotriene A4 synthase; LOG 5; LOG5; MGC163204; LOX5_HUMAN.
literatures	Specific References  (1)     |     SL3252R has been referenced in 1 publications. [IF=2.866] Xuan, Wenting. et al. Propofol Protects Against Erastin-Induced Ferroptosis in HT-22 Cells. J MOL NEUROSCI. 2022 Jun;:1-12  IF, WB ;  Mouse.   PubMed:35727524
Product Type	Phosphorylated anti
Research Area	Tumour  immunology  Signal transduction  transcriptional regulatory factor  Kinases and Phosphatases  Synthesis and Degradation
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	78kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from rat 5-Lipoxygenase around the phosphorylation site of Ser663: YL(p-S)PD
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. Function: Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes. Subunit: Interacts with ALOX5AP and LTC4S. Subcellular Location: Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Post-translational modifications: Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus. Similarity: Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain. SWISS: P09917 Gene ID: 25290 Database links: Entrez Gene: 477753 Dog Entrez Gene: 240 Human Entrez Gene: 11689 Mouse Entrez Gene: 25290 Rat Omim: 152390 Human SwissProt: P09917 Human SwissProt: P48999 Mouse SwissProt: P12527 Rat Unigene: 89499 Human Unigene: 41072 Mouse Unigene: 9662 Rat 5-脂氧合酶蛋白(5-LOX; 5-lipoxygenase; Arachidonate 5-lipoxygenase; 5-LO; ALOX5 )是花生四烯酸代谢途径中的一种关键酶,在恶性Tumour的发生、发展过程中起了重要作用.抑制5-脂氧合酶及其产物的表达有可能预防和逆转恶性Tumour的发生。
Product Picture	Sample: Heart (Rat) Lysate at 40 ug Primary: Anti- Phospho-5-Lipoxygenase (Ser663) (SL3252R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 78 kD Observed band size: 75 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-5-Lipoxygenase (Ser663)) Polyclonal Antibody, Unconjugated (SL3252R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-5-Lipoxygenase (Ser663)) Polyclonal Antibody, Unconjugated (SL3252R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Phospho-5-Lipoxygenase(Ser663) Polyclonal Antibody, Unconjugated(SL3252R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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