Product Name	Phospho-IRF7 (Ser471 + Ser472)
Chinese Name	磷酸化Interferon调节因子7抗体
Alias	Interferon regulatory factor 7; Interferon regulatory factor 7H; IRF 7; IRF 7A; IRF 7H; IRF7A; IRF7; IRF7H.
literatures	Specific References  (8)     |     SL3196R has been referenced in 8 publications. [IF=8.786] HuiDan Deng. et al. Mfn2 is responsible for inhibition of the RIG-I/IRF7 pathway and activation of NLRP3 inflammasome in Seneca Valley virus-infected PK-15 cells to promote viral replication. FRONT IMMUNOL. 2022; 13: 955671  WB ;  Pig.   PubMed:35958608 [IF=8.14] Han, Young Woo, et al. " Distinct Dictation of Japanese Encephalitis Virus-Induced Neuroinflammation and Lethality via Triggering TLR3 and TLR4 Signal Pathways." PLoS Pathogens 10(9) (2014): e103882.  WB ;  Mouse.   PubMed:25188232 [IF=6.286] Jianhao Yang. et al. Serum amyloid A regulates TLR2/4-mediated IFN-β signaling pathway against Marek's disease virus. VIRUS RES. 2023 Jan;:199044  WB ;  Chicken.   PubMed:36652973 [IF=5.64] Peng K. et al. Transcriptome Analyses of Senecavirus A-Infected PK-15 Cells: RIG-I and IRF7 Are the Important Factors in Inducing Type III Interferons.. Front Microbiol. 2022 Mar;13:846343-846343  WB ;  Pig.   PubMed:35308346 [IF=4.36] Menasria et al. Both TRIF and IPS-1 adaptor proteins contribute to the cerebral innate immune response against herpes simplex virus 1 infection. (2013) J.Virol. 87:7301-8  WB ;  Mouse.   PubMed:23596298 [IF=4.122] Lei Q et al. HEV ORF3 downregulates TLR7 to inhibit the generation of type I interferon via impairment of multiple signaling pathways Q Lei, L Li, S Zhang, T Li, X Zhang, X Ding, B Qin - Scientific Reports, 2018Sci Rep. 2018 Jun 5;8(1):8585.  WB ;  Human.   PubMed:29872132 [IF=3.23] Le Bel, Manon, and Jean Gosselin. "Leukotriene B 4 Enhances NOD2-Dependent Innate Response against Influenza Virus Infection." PloS one 10.10 (2015): e0139856.  WB ;  Mouse.   PubMed:26444420 [IF=2.8] Egarnes, Benoit, Marie-Renée Blanchet, and Jean Gosselin. "Treatment with the NR4A1 agonist cytosporone B controls influenza virus infection and improves pulmonary function in infected mice." PLOS ONE 12.10 (2017): e0186639.  WB ;  Mouse.   PubMed:29053748
Product Type	Phosphorylated anti
Research Area	Tumour  Signal transduction  Apoptosis  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Pig, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 ICC=1:100 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	54kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human IRF7 around the phosphorylation site of Ser471/472: GV(p-S)(p-S)LD
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established. [provided by RefSeq, Jul 2008] Function: Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) in IFN promoters and in the Q promoter (Qp) of EBV nuclear antigen 1 (EBNA1). Functions as a molecular switch for antiviral activity. Activated by phosphorylation in response to infection. Activation leads to nuclear retention, DNA binding, and derepression of transactivation ability. Subunit: Homodimer. Subcellular Location: Nucleus. Cytoplasm. The phosphorylated and active form accumulates selectively in the nucleus. Tissue Specificity: Expressed predominantly in spleen, thymus and peripheral blood leukocytes. Post-translational modifications: In response to a viral infection, phosphorylated on the C-terminal serine cluster. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3. TRAF6-mediated ubiquitination is required for IRF7 activation. Similarity: Belongs to the IRF family. Contains 1 IRF tryptophan pentad repeat DNA-binding domain. SWISS: Q92985 Gene ID: 3665 Database links: Entrez Gene: 3665 Human Entrez Gene: 54123 Mouse Entrez Gene: 293624 Rat Omim: 605047 Human SwissProt: Q92985 Human SwissProt: P70434 Mouse Unigene: 166120 Human Unigene: 3233 Mouse Unigene: 101159 Rat
Product Picture	Sample: Uterus (Mouse) Lysate at 40 ug Large intestine (Mouse) Lysate at 40 ug Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(SL3196R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 49kD Sample: Lane 1: Mouse Spleen tissue lysates Lane 2: Mouse Thymus tissue lysates Lane 3: Mouse Blood cell lysates Lane 4: Mouse Lung tissue lysates Lane 5: Rat Spleen tissue lysates Lane 6: Rat Liver tissue lysates Lane 7: Human Jurkat cell lysates Lane 8: Human Raji cell lysates Primary: Anti-Phospho-IRF7 (Ser471 + Ser472) (SL3196R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 70,55 kD Sample:Cerebrum (Rat) Lysate at 40 ug Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(SL3196R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 48kD Sample:Spleen (Mouse) Lysate at 40 ug Primary: Anti-Phospho-IRF7 (Ser471 + Ser472)(SL3196R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54kD Observed band size: 49kD Sample: Heart(Mouse) lysate at 30ug; Primary: Anti-Phospho-IRF7 (Ser471+Ser472) (SL3196R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size : 54kD Observed band size : 51kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (SL3196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) Polyclonal Antibody, Unconjugated (SL3196R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-IRF7 (Ser471 + Ser472)) polyclonal Antibody, Unconjugated (SL3196R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control:Molt-4. Primary Antibody (green line): Rabbit Anti-Phospho-IRF7 (Ser471 + Ser472) antibody (SL3196R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control:Mouse spleen. Primary Antibody (green line): Rabbit Anti-Phospho-IRF7 (Ser471 + Ser472) antibody (SL3196R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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