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Product Name phospho-Histone H1.4 (Thr18) Chinese Name 磷酸化组蛋白H1.4抗体 Alias Histone H1.4 (phospho T18); p-Histone H1.4 (phospho T18); HIST1H1E; H1F4; Histone H1.4; Histone H1b; H14_HUMAN; Histone H1s-4; H1 histone family member 4; H1E; Hist1h1e; Histone 1 H1e; Histone cluster 1 H1e; Histone H1; MGC116819; H1.4; H1F0; Histone H1b. Product Type Phosphorylated anti Research Area Tumour Cell biology Developmental biology Signal transduction transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 22kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human Histone H1.4 around the phosphorylation site of Thr18: EK(p-T)P Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Histone H1b are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Function:
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
Subcellular Location:
Nucleus. Chromosome.
Post-translational modifications:
Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
Similarity:
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain.
SWISS:
P10412
Gene ID:
3008
Database links:Entrez Gene: 3008 Human
Entrez Gene: 50709 Mouse
Omim: 142220 Human
SwissProt: P10412 Human
SwissProt: P43274 Mouse
Unigene: 248133 Human
Unigene: 170587 Mouse
Product Picture Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-Histone H1.4(Thr18) Polyclonal Antibody, Unconjugated(SL3171R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Histone H1.4 (Thr18)) Polyclonal Antibody, Unconjugated (SL3171R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Histone H1.4 (Thr18)) Polyclonal Antibody, Unconjugated (SL3171R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Histone H1.4 (Thr18)) Polyclonal Antibody, Unconjugated (SL3171R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Histone H1.4 (Thr18)) Polyclonal Antibody, Unconjugated (SL3171R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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