Product Name	phospho-GCN2 (Thr667)
Chinese Name	磷酸化蛋白激酶GCN2蛋白抗体
Alias	GCN2 (phospho T667); p-GCN2 (phospho T667); EIF2AK4; Eukaryotic Translation Initiation Factor 2-alpha kinase 4; GCN2-like protein; KIAA1338; E2AK4_HUMAN; Eif2ak4; Eukaryotic Translation Initiation Factor 2 alpha kinase 4; GCN2; GCN2 eIF2alpha kinase; GCN2-like protein; MGCN2.
Product Type	Phosphorylated anti
Research Area	Cell biology  Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Dog, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	181kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human GCN2 around the phosphorylation site of Thr667: PG(p-T)PP
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses. Tissue Specificity: Widely expressed. Post-translational modifications: Autophosphorylated on threonine residues. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. Contains 2 protein kinase domains. Contains 1 RWD domain. SWISS: Q9P2K8 Gene ID: 440275 Database links: Entrez Gene: 440275 Human Entrez Gene: 27103 Mouse Entrez Gene: 114859 Rat Omim: 609280 Human SwissProt: Q9P2K8 Human SwissProt: Q9QZ05 Mouse Unigene: 656673 Human Unigene: 217616 Mouse Unigene: 8333 Rat GCN2蛋白的作用；可能是将短期记忆变成长期记忆的一个主控调节因子， GCN2蛋白在长期记忆形成中功能的发现将可能帮助研究人员开发出提高记忆力丧失的病人提高记忆力。
Product Picture	Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Hippocampus (Mouse) Lysate at 40 ug Cerebral cortex (Mouse) Lysate at 40 ug Primary: Anti- phospho-GCN2 (Thr667) (SL3156R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 181 kD Observed band size: 170/181 kD Sample: Hippocampus (Mouse) Lysate at 40 ug Cerebral cortex (Mouse) Lysate at 40 ug Primary: Anti- phospho-GCN2 (Thr667) (SL3156R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 181 kD Observed band size: 170 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-GCN2 (Thr667)) Polyclonal Antibody, Unconjugated (SL3156R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-GCN2 (Thr667)) Polyclonal Antibody, Unconjugated (SL3156R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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