Product Name	Phospho-DARPP32 (Thr75)
Chinese Name	磷酸化多巴胺/cAMP调节磷蛋白抗体
Alias	DARPP32 (phospho T75); p-DARPP32 (phospho T75); PPP1R1B(phospho T75); DARPP 32; Dopamine and cAMP regulated neuronal phosphoprotein; Dopamine and cAMP regulated phosphoprotein DARPP 32; Dopamine and cAMP regulated phosphoprotein DARPP32; FLJ20940; IPPD; Neuronal phosphoprotein DARPP 32; PPP1R1B; Protein phosphatase 1 regulatory (inhibitor) subunit 1B; Protein phosphatase 1 regulatory subunit 1B; PPR1B_HUMAN.
Product Type	Phosphorylated anti
Research Area	Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Guinea Pig,  (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	32kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human DARPP32 around the phosphorylation site of Thr75: AY(p-T)PP
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]. Function: Inhibitor of protein-phosphatase 1. Subcellular Location: Cytoplasm. Post-translational modifications: Phosphorylation of Thr-34 is required for activity. Similarity: Belongs to the protein phosphatase inhibitor 1 family. SWISS: Q9UD71 Gene ID: 84152 Database links: Entrez Gene: 84152 Human Entrez Gene: 19049 Mouse Entrez Gene: 360616 Rat Omim: 604399 Human SwissProt: Q9UD71 Human SwissProt: Q60829 Mouse SwissProt: Q6J4I0 Rat Unigene: 286192 Human Unigene: 45372 Mouse Unigene: 70366 Rat     DARPP-32通过自身不同位点的磷酸化对蛋白质磷酸化和去磷酸化过程发挥着双向调控的作用.DARRP-32在脑内存在于接受多巴胺能投射的神经元中,其磷酸化过程受多种神经递质的调控,在Signal transduction途径中处于中心位置,整合多种胞内Signal transduction,调节神经元的电学和化学性质以及动物的生理行为反应,参与多种生理功能和病理过程,包括药物成瘾、抑郁症、精神分裂症等.此外,DARPP-32在非神经系统的功能也逐渐被人们所认识.     多巴胺和cAMP调节的磷蛋白(dopamine and adenosine 3'5'-monophosphate-regulated phospho-protein,Mr 32 kD,DARPP-32)是一个具有双重功能的独特蛋白,它既是磷酸酶(如PP-1)的抑制剂,也是蛋白激酶(如PKA)的抑制剂。
Product Picture	Sample: Cerebral cortex (Mouse) Lysate at 40 ug Primary: Anti- Phospho-DARPP32 (Thr75) (SL3119R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-DARPP32 (Thr75)) Polyclonal Antibody, Unconjugated (SL3119R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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