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Product Name Phospho-DOK2 (Tyr351) Chinese Name 磷酸化D酪氨酸激酶衰减蛋白2抗体 Alias DOK2 (Phospho-Tyr351); DOK2 (Phospho Tyr351); DOK2 (Phospho Y351); Docking protein 2 56kDa; Docking protein 2 antibody DOK 2; DOK R; Downstream of tyrosine kinase 2; p56(dok 2); p56dok 2; p56DOK; p56dok2; DOK2; DOK2_HUMAN; Downstream of tyrosine kinase 2; OTTHUMP00000161710; OTTHUMP00000224923; p56(dok 2); p56(dok-2). Product Type Phosphorylated anti Research Area Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 45kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human p56Dok2 around the phosphorylation site of (Tyr351): HI(p-Y)DE Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq, Jul 2008]
Function:
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.
Subunit:
Interacts with phosphorylated RASGAP and EGFR. Interacts with RET and NCK. Interacts (via PH domain) with TEK/TIE2 (tyrosine phosphorylated).
Tissue Specificity:
Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
Post-translational modifications:
On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP.
Similarity:
Belongs to the DOK family. Type A subfamily.
Contains 1 IRS-type PTB domain.
Contains 1 PH domain.
SWISS:
O70469
Gene ID:
9046
Database links:Entrez Gene: 9046 Human
Entrez Gene: 13449 Mouse
Omim: 604997 Human
SwissProt: O60496 Human
SwissProt: O70469 Mouse
Unigene: 71215 Human
Unigene: 243323 Mouse
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