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Product Name Phospho-Cofilin (Ser3) Chinese Name 磷酸化丝切蛋白(Ser3)抗体 Alias Cofilin (Phospho-Ser3); Cofilin (Phospho S3); 18 kDa phosphoprotein; CFL 1; CFL; CFL1; Cofilin 1; Cofilin 1 non muscle; Cofilin non muscle isoform; p18; COF1_HUMAN. literatures Specific References (3) | SL3099R has been referenced in 3 publications.[IF=4.014] Nuo Heng. et al. RhoA improves cryopreservation of rooster sperm through the Rho/RhoA-associated Kinase/cofilin pathway. POULTRY SCIENCE. 2022 Jul;:102051 WB ; Chicken.[IF=2.894] Wufuer R et al. Downregulation of Rac1/PAK1/LIMK1/cofilin signaling pathway in colon cancer SW620 cells treated with Chlorin e6 photodynamic therapyPhotodiagnosis Photodyn Ther.2020 Dec 8;102143. WB ; Human.[IF=2.705] Xue Y et al. Methamphetamine reduces expressions of tight junction proteins, rearranges F-actincytoskeleton and increases the blood brain barrier permeability via the RhoA/ROCK-dependent pathway. Biochem Biophys Res Commun. 2019 Feb 5;509(2):395-401. WB ; Rat.Product Type Phosphorylated anti Research Area Tumour Cell biology immunology Neurobiology Signal transduction Growth factors and hormones Cell adhesion molecule Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Pig, Cow, Sheep, ) Applications WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 18kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human Cofilin around the phosphorylation site of Ser3: A(p-S)GV Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004].
Function:
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Subunit:
Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods.
Subcellular Location:
Nucleus matrix. Cytoplasm, cytoskeleton.
Tissue Specificity:
Widely distributed in various tissues.
Post-translational modifications:
Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells. Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
Similarity:
Belongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain.
SWISS:
P23528
Gene ID:
1072
Database links:Entrez Gene: 1072 Human
Entrez Gene: 12631 Mouse
Entrez Gene: 494432 Sheep
Omim: 601442 Human
SwissProt: P23528 Human
SwissProt: P18760 Mouse
SwissProt: Q6B7M7 Sheep
Unigene: 52428 Cow
Unigene: 170622 Human
Unigene: 329655 Mouse
Unigene: 11675 Rat
Unigene: 13212 Sheep
丝切蛋白(cofilin)是Cytoskeleton塑形的重要调节因素,其主要功能是分解肌动蛋白微丝和增加肌动蛋白单体从肌动蛋白微丝的末端解离的速度,从而促进肌动蛋白微丝的循环,是目前为止已发现的肌动蛋白Binding protein家族中唯一能够改变肌动蛋白微丝扭曲结构的蛋白.Product Picture Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Phospho-Cofilin (Ser3) (SL3099R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 18 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Cofilin (Ser3)) Polyclonal Antibody, Unconjugated (SL3099R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Cofilin (Ser3)) Polyclonal Antibody, Unconjugated (SL3099R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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