Product Name	phospho-CaMK2 alpha (Tyr230)
Chinese Name	磷酸化钙/钙调素依赖蛋白激酶2α抗体
Alias	phospho-CaMK2B (Tyr230); CaMK2B (phospho Tyr230); alpha CaMKII; CaMKII alpha; Calcium / Calmodulin Dependent Protein Kinase II G; Calcium calmodulin dependent protein kinase CaM kinase II alpha; Calcium calmodulin dependent protein kinase II alpha-B subunit; Calcium calmodulin dependent protein kinase II; Calcium/calmodulin dependent protein kinase II alpha; Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; Calcium/calmodulin-dependent protein kinase II alpha; Calcium/calmodulin-dependent protein kinase II-alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type IIA; CaM kinase II alpha chain; CaM kinase II alpha subunit; CaM kinase II beta chain; CaM kinase II beta subunit; CaM kinase II delta chain; CaM kinase II delta subunit; CaM kinase II subunit alpha; CAM2; CaMK II alpha subunit; CaMK II beta subunit; CaMK II delta subunit; CaMK-II subunit alpha; CAMK2A; CAMK2B; CAMK2D; CAMKA; CAMKB; CaMKII; EC 2.7.11.17; KIAA0968; MGC123320; MGC139375; MGC155201; mKIAA0968; PK2CDD; PKCCD; R74975; zgc:112538; zgc:123320; KCC2A_HUMAN.  phospho-CaMK2α (Tyr230); p-CaMK2α (Tyr230);
literatures	Specific References  (4)     |     SL3081R has been referenced in 4 publications. [IF=8.679] Qi Sun. et al. MORTALIN-Ca2+ axis drives innate rituximab resistance in diffuse large B-cell lymphoma. CANCER LETT. 2022 Jul;537:215678  WB ;  Human.   PubMed:35447282 [IF=4.994] He Zhang. et al. Effect of feeding regimen on circadian activity rhythms of food anticipatory by ghrelin hormone in a pig model. 2022 Mar 05  WB ;  Pig.   PubMed:35249475 [IF=3.943] Henrique Ballassini Abdalla et al. Activation of PPAR-γ induces macrophage polarization and reduces neutrophil migration mediated by heme oxygenase 1. Int Immunopharmacol. 2020 Jul;84:106565.  WB ;  Rat.   PubMed:32388213 [IF=2.733] Bei Miao. et al. Protective effect of HCN2-induced SON sensitization on chronic visceral hypersensitivity in neonatal-CRD rat model. Brain Res. 2021 Sep;1767:147538  WB ;  Rat.   PubMed:34052259
Product Type	Phosphorylated anti
Research Area	Cell biology  immunology  Signal transduction  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	54kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human CaMK2 alpha around the phosphorylation site of Tyr231: GA(p-Y)DF
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Ca2+/CalmodulinDependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium and calmodulindependent protein kinase that mediates cellular responses to a wide variety of intercellular signals. CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+homeostasis. Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II. Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning. Function: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. Subunit: CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with BAALC, MPDZ, SYN1, CAMK2N2 and SYNGAP1. Subcellular Location: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Tissue Specificity: Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle. Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain. SWISS: Q9UQM7 Gene ID: 815 Database links: Entrez Gene: 815 Human Entrez Gene: 12322 Mouse Entrez Gene: 25400 Rat Omim: 114078 Human SwissProt: Q9UQM7 Human SwissProt: P11798 Mouse SwissProt: P11275 Rat Unigene: 725992 Human Unigene: 131530 Mouse Unigene: 107499 Rat
Product Picture	Sample: Cerebrum(Rat)Cell Lysate at 40 ug Primary: Anti-phospho-CaMK2 alpha (Tyr230) (SL3081R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD Sample: Lane 1: Mouse Muscle tissue lysates Lane 2: Mouse Heart tissue lysates Primary: Anti-phospho-CaMK2 alpha (Tyr230) (SL3081R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 50 kDa Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-CaMK2 alpha) Polyclonal Antibody, Unconjugated (SL3081R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human gastric ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-CaMK2 alpha(Tyr230)) Polyclonal Antibody, Unconjugated (SL3081R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human kidney ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-CaMK2 alpha(Tyr230)) Polyclonal Antibody, Unconjugated (SL3081R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-CaMK2 alpha(Tyr230)) Polyclonal Antibody, Unconjugated (SL3081R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control: K562. Primary Antibody (green line): Rabbit Anti-phospho-CaMK2 alpha (Tyr230) antibody (SL3081R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control: K562. Primary Antibody (green line): Rabbit Anti-phospho-CaMK2 alpha (Tyr230) antibody (SL3081R) Dilution: 2ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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