Product Name	Phospho-CRMP2 (Thr514)
Chinese Name	磷酸化二氢嘧啶酶样2(CRMP2/DPYL2)抗体
Alias	CRMP2 (phospho T514); p-CRMP2 (phospho T514); CRMP2 (phospho T514); p-CRMP2 (phospho T514); CRMP2 (phospho T514); CRMP-2(phospho Thr514); p-CRMP2(Thr514); Collapsin response mediator protein 2; Collapsin response mediator protein hCRMP 2; CRAM; CRMP 2; CRMP-2; CRMP2; DHPRP 2; DHPRP2; Dihydr pyrimidinase 2; Dihydropyrimidinase 2; Dihydropyrimidinase like 2; Dihydropyrimidinase like 2 long form; Dihydropyrimidinase related protein 2; Dihydropyrimidinase-related protein 2; DPYL 2; DPYL2; DPYL3_HUMAN; DPYSL 2; Dpysl2; DRP 2; DRP-2; DRP2; Musunc 33; Musunc33; N2A3; TOAD 64; TOAD64; ULIP 2 protein; ULIP-2; Ulip2; Unc-33-like phosphoprotein 2.
literatures	Specific References  (2)     |     SL3068R has been referenced in 2 publications. [IF=3.74] Zheng, Jian, et al. "Lithium posttreatment confers neuroprotection through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats." Journal of Neurosurgery (2016): 1-9.  WB ;  Rat.   PubMed:27739937 [IF=3.04] Liu Z et al. The neuroprotective effect of lithium chloride on cognitive impairment through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats.(2018) Eur J Pharmacol. 2018 Dec 5;840:50-59.  WB ;  Rat.   PubMed:30336136
Product Type	Phosphorylated anti
Research Area	Cell biology  immunology  Developmental biology  Neurobiology  Growth factors and hormones
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	62kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human CRMP-2 around the phosphorylation site of Thr514: TV(p-T)PA
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins involved in neuronal differentiation and axonal guidance. CRMP2 was previously shown to mediate the repulsive effect of Sema3A on axons and to participate in axonal specification. The CRMPs appear to play a complex role in axon growth as well as microtubule dynamics and axon induction. CRMPs localize to the lamellipodia and filopodia of axonal growth cones, suggesting a role in axon guidance. Moreover, CRMP2 is upregulated after axotomy, and appears to increase the formation of axon-type processes from hippocampal neurons. CRMP2 has been reported to bind tubulin dimers directly and modulate microtubule assembly. CRMPs have also been implicated in the pathogenesis of a paraneoplastic neurologic syndrome. Interaction studies have implicated phospholipase D2 (PLD2), the cytosolic tyrosine kinase Fes, and intersectin in CRMP function. Hyperphosphorylation of CRMP2 is an early event in the progression of Alzheimer's disease. Function: Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration (By similarity). Subunit: Homotetramer, and heterotetramer with CRMP1, DPYSL2, DPYSL4 or DPYSL5 (By similarity). Interacts with synaptic vesicle protein 2 and SH3A domain of intersectin (By similarity). Subcellular Location: Cytoplasm. Cell projection, growth cone. Note=Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone. Tissue Specificity: Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord. Post-translational modifications: Phosphorylation on Ser-522 by DYRK2 promotes subsequent phosphorylation on Thr-509, Thr-514 and Ser-518 by GSK3. Similarity: Belongs to the DHOase family. SWISS: Q16555 Gene ID: 1808 Database links: Entrez Gene: 1808 Human Entrez Gene: 12934 Mouse Entrez Gene: 25416 Rat Omim: 602463 Human SwissProt: Q16555 Human SwissProt: O08553 Mouse SwissProt: P47942 Rat Unigene: 173381 Human Unigene: 352648 Mouse Unigene: 475100 Mouse Unigene: 2889 Rat
Product Picture	Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-Phospho-CRMP2 (Thr514) (SL3068R) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 65 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Phospho-CRMP2 (Thr514)) Polyclonal Antibody, Unconjugated (SL3068R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Blank control:Molt-4. Primary Antibody (green line): Rabbit Anti-Phospho-CRMP2 (Thr514) antibody (SL3068) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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