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Product Name Phospho-CEBP alpha (Thr222 + Thr226) Chinese Name 磷酸化transcriptional regulatory factorCEBP α 抗体 Alias CEBP Alpha (phospho T226); p-CEBP Alpha (phospho T226); CEBP Alpha (phospho T222); p-CEBP Alpha (phospho T222); Apoptotic cysteine protease; Apoptotic protease Mch 5; C/EBP alpha; CAP4; Caspase 8 precursor; CCAAT Enhancer Binding Protein alpha; CEBP; CEBP A; CEBP alpha; CEBPA; FADD homologous ICE/CED 3 like protease; FADD like ICE; FLICE; ICE like apoptotic protease 5; ICE8; MACH; MCH5; MORT1 associated CED 3 homolog; CEBPA_HUMAN. Product Type Phosphorylated anti Research Area Cell biology immunology transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 38kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human CEBP alpha around the phosphorylation site of Thr222/226: HP(p-T)PP Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Mutations in this gene are frequently found in acute myeloid leukemia (AML).
Function:
C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
Subunit:
Interacts with PRDM16 (By similarity). Binds DNA as a dimer and can form stable heterodimers with C/EBP beta and gamma. Interacts with UBN1. Interacts with HBV protein X. Interacts with ZNF638; this interaction increases transcriptional activation.
Subcellular Location:
Nucleus.
Similarity:
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain.
SWISS:
P49715
Gene ID:
1050
Database links:Entrez Gene: 1050 Human
Entrez Gene: 12606 Mouse
Omim: 116897 Human
SwissProt: P49715 Human
SwissProt: P53566 Mouse
Unigene: 76171 Human
Unigene: 349667 Mouse
Unigene: 153603 Rat
Unigene: 204833 Rat
Product Picture Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human MCF-7 cell lysates
Primary: Anti-Phospho-CEBP alpha (Thr222 + Thr226) (SL3061R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 44 kDa
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEBPA) Polyclonal Antibody, Unconjugated (SL3061R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEBPA) Polyclonal Antibody, Unconjugated (SL3061R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEBPA) Polyclonal Antibody, Unconjugated (SL3061R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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