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Product Name RECK Chinese Name 金属蛋白酶抑制因子RECK抗体 Alias hRECK; Membrane anchored glycoprotein (metastasis and invasion); RECK protein; Reversion inducing cysteine rich protein with Kazal motifs; ST15; Suppression of tumorigenicity 15 (reversion inducing cysteine rich protein with kazal motifs); Suppressor of tumorigenicity 15; RECK_HUMAN. literatures Specific References (4) | SL2901R has been referenced in 4 publications.[IF=4.22] Siddesha, Jalahalli M., et al. ʺAcetylsalicylic Acid Inhibits IL‐18‐Induced Cardiac Fibroblast Migration through the Induction of RECK.ʺ Journal of cellular physiology (2013). Mouse.[IF=3.309] Liu L et al. Rab7 empowers renal tubular epithelial cells with autophagy-mediated protection against albumin-induced injury.Exp Cell Res. 2018 Sep 15;370(2):198-207. WB ; Rat&Human.[IF=2.511] Zhou L et al. The role of miR-21/RECK in the inhibition of osteosarcoma by curcumin. Mol Cell Probes. 2020 Feb 17:101534. WB ; Human.[IF=1.03] Liu, Jianfeng, et al. "RECK is involved in intimal hyperplasia in carotid arteries: A new insight in the prevention of restenosis after vascular angioplasty."Annals of Vascular Surgery (2015). WB ; Human.Research Area Tumour immunology Signal transduction Apoptosis transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 107kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human RECK: 801-900/971 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail RECK is a cysteine rich, extracellular protein with protease inhibitor like domains whose expression is suppressed strongly in many tumors and cells transformed by various kinds of oncogenes. In normal cells, this membrane anchored glycoprotein may serve as a negative regulator for matrix metalloproteinase 9, a key enzyme involved in tumor invasion and metastasis.
Function:
Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression.
Subunit:
Interacts with MMP-9.
Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Specificity:
Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells.
Post-translational modifications:
N-glycosylated.
Similarity:
Contains 3 Kazal-like domains.
SWISS:
O95980
Gene ID:
313488
Database links:Entrez Gene: 313488 Human
Entrez Gene: 8434 Human
Omim: 605227 Human
SwissProt: O95980 Human
SwissProt: Q5W0K6 Human
Unigene: 388918 Human
Unigene: 728961 Human
Product Picture Sample: Lane 1: Mouse Kidney tissue lysates Lane 2: Mouse NIH/3T3 cell lysates Lane 3: Human Huvec cell lysates Lane 4: Human U87MG cell lysates Primary: Anti-RECK (SL2901R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 107 kDa Observed band size: 120 kDaBlank control:U937.
Primary Antibody (green line): Rabbit Anti-RECK antibody (SL2901R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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