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Product Name VSTM1 Chinese Name V-Set 和含有跨膜结构域的蛋白质 1抗体 Alias V-Set And Transmembrane Domain Containing; UNQ3033; V-Set And Transmembrane Domain-Containing Protein 1; Signal Inhibitory Receptor On Leukocytes-1; SIRL-1; OSCAR-Like Transcript-1 ; LAIR Homolog ; SIRL1. Immunogen Species Rabbit Clonality Polyclonal React Species Rat, Applications WB=1:500-2000 IHC-P=1:400-800 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 24kDa Cellular localization The cell membrane Secretory protein Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from rat VSTM1: 111-209/209 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail VSTM1 (V-Set And Transmembrane Domain Containing 1) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include cytokine activity. An important paralog of this gene is TARM1.
Function:
Isoform 2: Behaves as a cytokine, promoting IL17A secretion by CD4+ T-cells, and differentiation and activation of IL17 producing helper T-cells (TH17). Isoform 1: Inhibitory immune receptor involved in the regulation of phagocytes.
Gene ID:
100360054
Product Picture Sample:
Lane 1: Rat Bone Lysates
Lane 2: Rat Spleen Lysates
Lane 3: Rat Lymph Lysates
Primary: Anti-VSTM1 (SL2861R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24kDa
Observed band size: 24kDa
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VSTM1) Polyclonal Antibody, Unconjugated (SL2861R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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