Product Name	KAT4/TAF1
Chinese Name	细胞周期基因1蛋白抗体
Alias	TBP Associated Factor 1; BA2R; CCG 1; CCG1; CCGS; Cell cycle G1 phase defect; Cell cycle gene 1 protein; Complementation of cell cycle block G1 to S; DYT3 protein; NSCL 2; NSCL2; OF; p250; TAF 1; TAF 2A; TAF(II)250; TAF1; TAF1 RNA polymerase II TATA box binding protein (TBP) associated factor 250kDa; TAF1 RNA polymerase II TATA box binding protein (TBP) associated factor; TAF1 RNA polymerase II TATA box binding protein associated factor 250kDa; TAF1_HUMAN; TAF2A; TAFII 250; TAFII-250; TAFII250; TAF II P250; TATA box binding protein (TBP) associated factor RNA polymerase II A; TATA box binding protein (TBP) associated factor RNA polymerase II A 250kD; TBP associated factor 250 kDa; TBP-associated factor 250 kDa; Transcription factor TFIID p250 polypeptide; Transcription initiation factor TFIID 250 kDa subunit; Transcription initiation factor TFIID subunit 1.
Research Area	Chromatin and nuclear signals  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Chicken, Dog, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	200kDa
Detection molecular weight	250-300 kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human TAF1: 501-600/1872
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. Function: Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. Subunit: TAF1 is the largest component of transcription factor TFIID that is composed of TBP and a variety of TBP-associated factors. TAF1, when part of the TFIID complex, interacts with C-terminus of TP53. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components C17orf49, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. RB1 interacts with the N-terminal domain of TAF1. Interacts with ASF1A and ASF1B. Interacts with SV40 Large T antigen. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated by casein kinase II in vitro. DISEASE: Defects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease. Similarity: Belongs to the TAF1 family. Contains 2 bromo domains. Contains 1 HMG box DNA-binding domain. Contains 2 protein kinase domains. SWISS: P21675 Gene ID: 6872 Database links: Entrez Gene: 6872 Human Entrez Gene: 270627 Mouse Entrez Gene: 317256 Rat Omim: 313650 Human SwissProt: P21675 Human SwissProt: Q80UV9 Mouse Unigene: 158560 Human Unigene: 722619 Human Unigene: 261750 Mouse
Product Picture	Sample: Lane 1: Human 293T cell lysates Lane 2: Human HeLa cell lysates Primary: Anti-KAT4/TAF1 (SL2812R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 200 kDa Observed band size: 300 kDa Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KAT4) Polyclonal Antibody, Unconjugated (SL2812R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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