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Product Name TNFAIP3 Chinese Name Tumour坏死因子α诱导蛋白3抗体 Alias Zinc finger protein A20; A20; TNFA1P2; TNFAIP 3; Tnfip 3; Tnfip3; Tumor necrosis factor alpha induced protein 3; Tumor necrosis factor induced protein 3; Tumor necrosis factor inducible protein A20; MGC104522; MGC138687; MGC138688; OTU domain containing protein 7C; OTUD7C; Putative DNA binding protein A20. literatures Specific References (1) | SL2803R has been referenced in 1 publications.[IF=4.741] Riffo-Vasquez Y et al. Modulation of allergic inflammation in the lung by a peptide derived from mycobacteria tuberculosis chaperonin 60.1. Clin Exp Allergy. 2019 Dec 16. IHC ; Mouse.Research Area Tumour Signal transduction Apoptosis Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Horse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 90kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TNFAIP3: 51-150/790 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail A20 is a Cys2/Cys2 zinc finger protein that is induced by a variety of inflammatory stimuli and regulates gene expression. Specifically, A20 is induced by tumor necrosis factor (TNF) and interleukin 1 (IL-1), and acts as a negative regulator of nuclear factor κ B (NFκB) gene expression. By inhibiting NFκB activation, A20 plays a critical role in terminating NFκB responses to various stimuli. Although the C-terminal region of A20 contains seven zinc finger domains, only four of these domains are required for in vitro inhibition of TNF-induced NFκB activation. A20 also interacts with several other proteins, such as TRAF2, TRAF6 and IkB kinase (IKK) γ protein, and can thereby inhibit cell death. TXBP151, a novel A20-binding protein, may mediate the anti-apoptotic activity of A20. Involved in the negative feedback regulation of signal transduction, A20 and A20-binding proteins may be useful as novel therapeutic tools in the treatment of a variety of diseases
Function:
TNFAIP3 interacts with NAF1 and inhibits TNF induced NF kappa B dependent gene expression by interfering with a RIP or TRAF2 mediated transactivation signal. It inhibits programmed cell death, has a role in the function of the lymphoid system, and may contribute to the in vivo effects of TNF.
Subcellular Location:
Cytoplasm. Nucleus.
Similarity:
Belongs to the peptidase C64 family.
Contains 7 A20-type zinc fingers.
Contains 1 OTU domain.
SWISS:
P21580
Gene ID:
7128
Database links:Entrez Gene: 7128 Human
Entrez Gene: 21929 Mouse
Omim: 191163 Human
SwissProt: P21580 Human
SwissProt: Q60769 Mouse
Unigene: 211600 Human
Unigene: 116683 Mouse
TNFAIP3是一种Ubiquitin修饰酶,是机体重要的炎症反应负性调节因子,也是NF-κB的负调节蛋白。TNFAIP3蛋白与很多变态反应疾病的病理生理演变相关联,如:包括类风湿关节炎、冠状动脉疾病、银屑病、腹腔疾病、I型Diabetes、炎症性肠炎和系统性红斑狼疮等。Product Picture Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNFAIP3) Polyclonal Antibody, Unconjugated (SL2803R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TNFAIP3) Polyclonal Antibody, Unconjugated (SL2803R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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