Product Name	phospho-Akt (Thr308)
Chinese Name	磷酸化蛋白激酶B抗体
Alias	AKT (phospho T308); p-AKT (phospho T308); Akt(Phospho-Thr308); Akt(Phospho T308); C AKT; MGC9965; Oncogene AKT1; PKB; PRKBA; Protein Kinase B Alpha; RAC Alpha; RAC; RAC PK Alpha; RAC Serine/Threonine Protein Kinase; vAKT Murine Thymoma Viral Oncogene Homolog 1; AKT1_HUMAN.
literatures	Specific References  (27)     |     SL2720R has been referenced in 27 publications. [IF=11.508] Qinyu Ma. et al. Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res. 2021 Jan;9(1):1-12  WB ;  Mouse.   PubMed:33431863 [IF=7.74] Li et al. The dual PI3K/mTOR inhibitor NVP-BEZ235 inhibits proliferation and induces apoptosis of burkitt lymphoma cells. (2015) Cancer.Cell.Int. 15:65  WB ;  Human.   PubMed:26130968 [IF=7.65] Wang Y et al. Targeting the miR-122/PKM2 autophagy axis relieves arsenic stress。 Journal of Hazardous Materials. 2019 Sep.  WB ;  Chicken.   PubMed:31546213 [IF=6.638] Wang Z et al. Light-activatable dual prodrug polymer nanoparticle for precise synergistic chemotherapy guidedby drug-mediated computed tomography imaging. Acta Biomater. 2019 May 22.  WB ;  Human.   PubMed:31128323 [IF=6.208] Yongxin Guo. et al. Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis. INT J MOL SCI. 2022 Jan;23(21):13152  WB ;  Rat, Human.   PubMed:36361940 [IF=5.715] Yu Wang. et al. Zinc application alleviates the adverse renal effects of arsenic stress in a protein quality control way in common carp. Environ Res. 2020 Dec;191:110063  WB ;  Fish.   PubMed:32818499 [IF=5.31] Ning Zhong. et al. Apatinib inhibits the growth of small cell lung cancer via a mechanism mediated by VEGF, PI3K/Akt and Ki-67/CD31. 2021 Sep 30  IF ;  mouse.   PubMed:34590406 [IF=4.85] Rui Sun. et al. Construction of crizotinib resistant models with CD74-ROS1 D2033N and CD74-ROS1 S1986F point mutations to explore resistance mechanism and treatment strategy. CELL SIGNAL. 2023 Jan;101:110497  WB ;  Human.   PubMed:36265718 [IF=4.85] Hui Tong. et al. Silencing of KNTC1 inhibits hepatocellular carcinoma cells progression via suppressing PI3K/Akt pathway. CELL SIGNAL. 2022 Oct;:110498  WB ;  Human.   PubMed:36273753 [IF=4.2] Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).  IHC-P ;  Mouse.   PubMed:29202267 [IF=4.2] Li Li. et al. Regulation of TREM2 on BV2 inflammation through PI3K/AKT/mTOR pathway. BIOTECHNOL GENET ENG. 2023 Apr 26  WB ;  Mouse.   PubMed:37125903 [IF=3.718] Qi Hao. et al. Taurine stimulates protein synthesis and proliferation of C2C12 myoblast cells through the PI3K-ARID4B-mTOR pathway. Brit J Nutr. 2021 Dec;:1-27  WB ;  Mouse.   PubMed:34881695 [IF=3.69] Jisheng Wang. et al. To study the mechanism of Cuscuta chinensis Lam. And Lycium barbarum L. in the treatment of asthenospermia based on network pharmacology. J Ethnopharmacol. 2021 Apr;270:113790  WB ;  Rat.   PubMed:33460759 [IF=3.34] Fan Zhiqin. et al. Phosphorylation of AKT by lysyl oxidase-like 2 activates the PI3K/AKT signaling pathway to promote proliferation, invasion and metastasis in esophageal squamous carcinoma. CLIN TRANSL ONCOL. 2023 Mar;:1-12  WB ;  Human.   PubMed:36995521 [IF=3.231] Mengqi Yu. et al. miR-27a Targeting PIK3R3 Regulates the Proliferation and Apoptosis of Sheep Hair Follicle Stem Cells. ANIMALS. 2023 Jan;13(1):141  WB ;  Sheep.   PubMed:36611750 [IF=3.216] Chao Lu. et al. Inhibition of Pre-B Cell Colony Enhancing Factor Reduces Lung Injury in Rats Receiving Cardiopulmonary Bypass. Drug Des Dev Ther. 2021; 15: 51–60  WB ;  Rat.   PubMed:33442236 [IF=3.043] Zheng R et al. Leucine attenuates muscle atrophy and autophagosome formation by activating PI3K/AKT/mTOR signaling pathway in rotator cuff tears. Cell Tissue Res. 2019 Apr 24.  WB ;  Rat.   PubMed:31020406 [IF=2.942] Li X et al. MicroRNA-33a negatively regulates myoblast proliferation by targeting IGF1, follistatin and cyclin D1. Biosci Rep. 2020 Jun 26;40(6):BSR20191327.  WB ;  Duck.   PubMed:32436962 [IF=2.794] Shi, Linjie. et al. Memory regulation in feeding habit transformation to dead prey fish of Chinese perch (Siniperca chuatsi). 2021 Sep 28  WB ;  Chinese perch.   PubMed:34581919 [IF=2.775] Ji-sheng Wang. et al. Explore the effects of pulmonary fibrosis on sperm quality and the role of the PI3K/Akt pathway based on rat model. 2021 Dec 21  WB ;  Rat.   PubMed:34932839 [IF=2.666] X Zhou et al. Modulating NMDA receptors to treat MK-801-induced schizophrenic cognition deficit: effects of clozapine combining with PQQ treatment and possible mechanisms of action. BMC Psychiatry. 2020 Mar 6;20(1):106.  WB ;  rat.   PubMed:32143671 [IF=2.532] Qi Zhao. et al. Investigation of the Underlying Mechanism of Depression on Male Infertility: A Bioinformatics and Experimental Research Study. ANDROLOGIA. 2023;2023:2820367  WB ;  Rat.   PubMed:10.1155/2023/2820367 [IF=2.311] Dezhi Wu. et al. Long non‑coding RNA maternally expressed gene 3 affects cell proliferation, apoptosis and migration by targeting the microRNA‑9‑5p/midkine axis and activating the phosphoinositide‑dependent kinase/AKT pathway in hepatocellular carcinoma. Oncol Lett. 2021 May;21(5):1-8  WB ;  Human.   PubMed:33747202 [IF=2.276] Wang Ji-sheng. et al. A Bioinformatic Investigation of the Mechanism Underlying Migraine-Induced Erectile Dysfunction. Biomed Res Int. 2021;2021:6674643  WB ;  Rat.   PubMed:33997039 [IF=2.16] Li, Xinxin, et al. "Long-term thermal manipulation in the late incubation period can inhibit breast muscle development by activating endoplasmic reticulum stress in duck (Anasplatyrhynchos domestica)." Journal of thermal biology 70.Pt B (2017): 37.  WB ;  Other Species.   PubMed:29108556 [IF=1.935] Ou X et al. Desumoylating Isopeptidase 2 (DESI2) Inhibits Proliferation and Promotes Apoptosis of Pancreatic Cancer Cells through Regulating PI3K/AKT/mTOR Signaling Pathway. (2018)Pathol. Oncol. Res. Nov 8.  WB ;  Human.   PubMed:30411297 [IF=1.785] Yu Zheng. et al. Advanced oxidation protein products trigger apoptosis and block epithelial‑to‑mesenchymal transition in crypt epithelial cells. Exp Ther Med. 2021 Aug;22(2):1-6  WB ;  Rat.   PubMed:34194563
Product Type	Phosphorylated anti
Research Area	Cell biology  Neurobiology  Signal transduction  Apoptosis  Cyclin  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	56kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Synthesised phosphopeptide derived from human Akt around the phosphorylation site of Thr308: MK(p-T)FC
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2020] Subunit: Interacts (via PH domain) with TCL1A; this enhances AKT3 phosphorylation and activation. Interacts with TRAF6. Subcellular Location: Cytoplasm. Nucleus. Cell membrane. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Tissue Specificity: Widely expressed. Low levels found in liver with slightly higher levels present in thymus and testis. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain. SWISS: P31749 Gene ID: 207 Database links: Entrez Gene: 207 Human Entrez Gene: 11651 Mouse Entrez Gene: 24185 Rat Omim: 164730 Human SwissProt: O57513 Chicken SwissProt: P31749 Human SwissProt: P31750 Mouse SwissProt: P47196 Rat Unigene: 525622 Human Unigene: 6645 Mouse Unigene: 11422 Rat
Product Picture	Sample: Liver (Mouse) Lysate at 30 ug Primary: Anti- phospho-Akt（Thr308）(SL2720R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution Predicted band size: 56 kD Observed band size: 56 kD Western blot analysis of extracts from Heart,using phospho-Akt(thr308) Antibody. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Akt (Thr308)) Polyclonal Antibody, Unconjugated (SL2720R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Akt (Thr308)) Polyclonal Antibody, Unconjugated (SL2720R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. Blank control (blue line): A431(Black). Primary Antibody (green line): Rabbit Anti-phospho-Akt (Thr308) antibody (SL2720R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE(Jackson lab) Dilution: 1μg /test. Protocol The cells were fixed with 4% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 20 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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