Product Name	GPNMB
Chinese Name	GPNMB抗体
Alias	NMB; Glycoprotein nmb like protein; Gpnmb; DC-HIL; Dchil; ipd; Glycoprotein (transmembrane) nmb; Glycoprotein nmb; HGFIN; Transmembrane glycoprotein; Transmembrane glycoprotein HGFIN; Transmembrane glycoprotein NMB; Osteoactivin; GPNMB_HUMAN.
literatures	Specific References  (9)     |     SL2684R has been referenced in 9 publications. [IF=6.847] Carmen Härdtner. et al. A comparative gene expression matrix in Apoe-deficient mice identifies unique and atherosclerotic disease stage-specific gene regulation patterns in monocytes and macrophages. ATHEROSCLEROSIS. 2023 Apr;371:1  IF ;  Mouse.   PubMed:36940535 [IF=5.17] Oyewumi et al. Osteoactivin (GPNMB) ectodomain protein promotes growth and invasive behavior of human lung cancer cells. (2016) Oncotarge. 7:13932-44  IHC ;  Human.   PubMed:26883195 [IF=5.1] Neal et al. The glycoprotein GPNMB attenuates astrocyte inflammatory responses through the CD44 receptor. (2018) J.Neuroinflammation. 15:73  IF(ICC) ;  Mouse.   PubMed:29519253 [IF=4.6] Abdelmagid, Samir M., et al. "Mutation in Osteoactivin Promotes RANKL-Mediated Osteoclast Differentiation and Survival, but Inhibits Osteoclast Function." Journal of Biological Chemistry (2015): jbc-M114.  WB ;  Mouse.   PubMed:25837253 [IF=4.183] Zhu Y et al. Protein Expression Profile in Rat Silicosis Model Reveals Upregulation of PTPN2 and Its Inhibitory Effect on Epithelial-Mesenchymal Transition by Dephosphorylation of STAT3. Int J Mol Sci. 2020 Feb 11;21(4).  WB ;  Rat&Mouse.   PubMed:32054021 [IF=3.84] Arosarena, Oneida A., et al. "Osteoactivin Promotes Migration of Oral Squamous Cell Carcinomas." Journal of Cellular Physiology (2016).  WB ;  Human.   PubMed:26636434 [IF=1.72] Frara, Nagat, et al. "Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping." BMC Musculoskeletal Disorders 17.1 (2016): 1.  WB ;  Rat.   PubMed:26781840 [IF=1.42] Ruan, Wei-Min, et al. "Differential expression of glycoprotein non-metastatic melanoma protein B (GPNMB) involved in trichostatin A-induced apoptosis in gastric cancer." International journal of clinical and experimental medicine 7.12 (2014): 4857-4866.  IHC-P ;  Human.   PubMed:25663982 [IF=0] Sondag, Gregory R. OSTEOACTIVIN AND CD44: A NOVEL INTERACTION REGULATING BONE CELL DIFFERENTIATION AND FUNCTION. Diss. Kent State University, 2015.  WB ;  Mouse.   PubMed:Diss.KentStateUniversity,2015.
Research Area	immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Pig, Cow, Horse, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	61kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GPNMB: 81-180/572 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: Could be a melanogenic enzyme. Subcellular Location: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue Specificity: Not restricted to the melanocytic lineage. Similarity: Belongs to the PMEL/NMB family. Contains 1 PKD domain. SWISS: Q14956 Gene ID: 10457 Database links: Entrez Gene: 10457 Human Entrez Gene: 93695 Mouse Entrez Gene: 113955 Rat Omim: 604368 Human SwissProt: Q14956 Human SwissProt: Q99P91 Mouse SwissProt: Q6P7C7 Rat Unigene: 190495 Human Unigene: 302602 Mouse Unigene: 13778 Rat
Product Picture	Sample: PANC-1(Human) Cell Lysate at 30 ug Primary: Anti-GPNMB (SL2684R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 61 kD Observed band size: 101 kD Tissue/cell: rat lung tissue(melanoma); 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩� for 20 min; Incubation: Anti-GPNMB/Osteoactivin Polyclonal Antibody, Unconjugated(SL2684R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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