Product Name	C5b-9
Chinese Name	末端补体复合物抗体
Alias	C5; C6; C7; C8; C9; Complement component 5; Complement component 6; Complement component 7; Complement component 8; Complement component 9; MAC; Membrane attack complex; TCC; Terminal complement complex.
literatures	Specific References  (11)     |     SL2673R has been referenced in 11 publications. [IF=19.456] Wenbin Yang. et al. IL-1β-dependent extravasation of preexisting lung-restricted autoantibodies during lung transplantation activates complement and mediates primary graft dysfunction. J CLIN INVEST. 2022 Oct 17;132(20):e157975  IHC ;  Mouse.   PubMed:36250462 [IF=9.685] Chen, Yujie. et al. GSDME deficiency leads to the aggravation of UVB-induced skin inflammation through enhancing recruitment and activation of neutrophils. CELL DEATH DIS. 2022 Oct;13(10):1-14  IF ;  Mouse.   PubMed:36182937 [IF=7.419] Keng Chen. et al. Complement factor B inhibitor LNP023 improves lupus nephritis in MRL/lpr mice. BIOMED PHARMACOTHER. 2022 Sep;153:113433  IHC, IF, WB ;  Mouse.   PubMed:10.1016/j.biopha.2022.113433 [IF=5.714] Hao-jie Zhang. et al. MAC mediates mammary duct epithelial cell injury in plasma cell mastitis and granulomatous mastitis. INT IMMUNOPHARMACOL. 2022 Dec;113:109303  WB ;  Human.   PubMed:36252469 [IF=5.156] Liu, Tao. et al. Microarray-based analysis of renal complement components reveals a therapeutic target for lupus nephritis. Arthritis Res Ther. 2021 Dec;23(1):1-15  IHC ;  Human.   PubMed:34433493 [IF=4.667] Zhao et al. Age-related increases in amyloid beta and membrane attack complex: evidence of inflammasome activation in the rodent eye. (2015) J.Neuroinflammation. 12:121  IHC ;  Rat.   PubMed:26104676 [IF=4.66] Ücal, Muammer, et al. "Widespread cortical demyelination of both hemispheres can be induced by injection of pro-inflammatory cytokines via an implanted catheter in the cortex of MOG-immunized rats." Experimental Neurology (2017).  IHC-P ;  Rat.   PubMed:28457906 [IF=4.64] Adzemovic, Milena Z., et al. "Efficacy of vitamin D in treating multiple sclerosis-like neuroinflammation depends on developmental stage." Experimental Neurology (2013).  IHC-P ;  Rat.   PubMed:23954214 [IF=3.548] Yang W et al. The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice. Clin Immunol. 2020 Jan 23;212:108350.  IHF-F ;  Mouse.   PubMed:31982645 [IF=3.221] Kenichi Serizawa. et al. Intradermal AQP4 peptide immunization induces clinical features of neuromyelitis optica spectrum disorder in mice. J NEUROIMMUNOL. 2023 Jul;380:578109  IHC ;  Mouse.   PubMed:37210799 [IF=2.567] Tsuge Mitsuru. et al. Neonatal hemochromatosis with εγδβ-thalassemia: a case report and analysis of serum iron regulators. BMC PEDIATR. 2022 Dec;22(1):1-7  IHC ;  Human.   PubMed:36309641
Research Area	Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	Purified Human SC5b-9 Complex
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Activation of the complement system plays a key role in normal inflammatory response to injury but may cause substantial injury when activated inappropriately. The complement system is activated either through the classical (antibody induced) or the alternative (microbial surface, polysacharride induced) pathway, both leading to the formation of the C5b9 complex. Fluid phase binding of the multifunctional glycoprotein S protein (vitronectin) to C5b9 leads to the formation of a cytolytically inactive complex, SC5b9, which is unable to attach to cells. SWISS: P02748 Gene ID: 727 Database links: Entrez Gene: 727 Human Entrez Gene: 729 Human Entrez Gene: 730 Human Entrez Gene: 731 Human Entrez Gene: 735 Human SwissProt: P01031 Human SwissProt: P02748 Human SwissProt: P07357 Human SwissProt: P10643 Human SwissProt: P13671 Human
Product Picture	Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug Lane 2: Hela (Human) Cell Lysate at 30 ug Primary: Anti-C5b (SL2673R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72 kD Observed band size: 72 kD Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C5b-9) Polyclonal Antibody, Unconjugated (SL2673R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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