Product Name	Regucalcin
Chinese Name	衰老标记蛋白30抗体
Alias	Gluconolactonase; GNL; RC; Regucalcin; RGN; RGN_HUMAN; Senescence marker protein 30; SMP 30; SMP-30.
Research Area	Cell biology  Signal transduction  Binding protein
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	33kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Regucalcin: 65-160/299
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Regucalcin is a Ca2+ binding protein that is encoded by a highly conserved gene in vertebrate species. Regucalcin is greatly expressed in liver and kidney cortex, and the expression is mediated through a Ca2+ signaling mechanism with AP1 and NFI-A1 as transcriptional factors. It plays a multifunctional role as a regulatory protein in intracellular signaling processes in the cytoplasm and nucleus of cells including the maintenance of intracellular Ca2+ homeostasis, the regulation of various Ca2+ dependent protein kinases and tyrosine kinases, protein phosphatases, nitric oxide (NO) synthase, and the suppression of nuclear DNA and RNA syntheses. Regucalcin has suppressive effects on cell proliferation, cell death and apoptosis, and is suggested to play a physiologic role in the maintenance of cellular homeostasis. Function: Gluconolactonase with low activity towards other sugar lactones, including gulonolactone and galactonolactone. Can also hydrolyze diisopropyl phosphorofluoridate and phenylacetate (in vitro). Calcium-binding protein. Modulates Ca(2+) signaling, and Ca(2+)-dependent cellular processes and enzyme activities. Subunit: Monomer. Subcellular Location: Cytoplasm. Similarity: Belongs to the SMP-30/CGR1 family. SWISS: Q15493 Gene ID: 9104 Database links: Entrez Gene: 9104 Human Omim: 300212 Human SwissProt: Q15493 Human SwissProt: Q5JRR5 Human Unigene: 77854 Human
Product Picture	Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-Regucalcin (SL2665R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 33 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Regucalcin) Polyclonal Antibody, Unconjugated (SL2665R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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