Product Name	IL-3
Chinese Name	白介素3抗体
Alias	Colony stimulating factor multiple; Hematopoietic growth factor; IL 3; IL3; IL3_HUMAN; IL3_MOUSE; Interleukin 3 (colony stimulating factor, multiple); Interleukin 3; Interleukin-3; Mast cell growth factor; MCGF; MGC79398; MGC79399; Multi CSF; Multilineage colony stimulating factor; Multipotential colony stimulating factor; Multipotential colony-stimulating factor; OTTHUMP00000065963; P cell stimulating factor; P-cell-stimulating factor
Research Area	Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Rat, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	16kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from mouse IL-3: 101-166/166
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The pleiotropic IL3 (Interleukin 3) is a 15kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL3 has also been shown to protect mast cells from undergoing apoptosis. Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. Subunit: Monomer. Subcellular Location: Secreted. Tissue Specificity: Activated T-cells, mast cells, natural killer cells. Similarity: Belongs to the IL-3 family. SWISS: P01586 Gene ID: 16187 Database links: Entrez Gene: 3562 Human Entrez Gene: 16187 Mouse Omim: 147740 Human SwissProt: P08700 Human SwissProt: P01586 Mouse Unigene: 694 Human Unigene: 983 Mouse
Product Picture	Sample: Lung(Mouse) lysate at 30ug; Primary: Anti- IL3 (SL2598R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size:16 kD Observed band size:16 kD Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: EDTA( 0.01M, pH 9.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-3 Polyclonal Antibody, Unconjugated(SL2598R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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