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Product Name IL-1R2 Chinese Name 白介素1受体2抗体 Alias IL1 Receptor II; IL-1R II; IL1 Receptor 2; interleukin-1 receptor type 2 precursor; IL1R2; CD121b; IL1RB; CD121; CD121 antigen-like family member B; CD121b; CD121B Antigen; CDW121B; IL-1R-2; IL-1R-beta; IL-1R2; IL-1RT-2; IL-1RT2; IL1R Beta; Il1r-2; IL1R2; IL1RB; Interleukin 1 receptor, beta, type II; Interleukin 1 receptor, type II; Interleukin-1 receptor beta; Interleukin-1 receptor type 2; MGC137383; MGC47725; p68; IL1 Receptor II; IL1R2_HUMAN. Research Area immunology Stem cells lymphocyte b-lymphocyte Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Dog, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 44kDa Cellular localization The cell membrane Extracellular matrix Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human IL-1R II: 301-398/398 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4(IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Two alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq].
Function:
Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
Subcellular Location:
Isoform Short: Secreted.
Isoform Long: Cell membrane; Single-pass type I membrane protein.
Post-translational modifications:
A soluble form (sIL1R2) can also be produced by proteolytic cleavage at the cell surface (shedding) involving a metalloproteinase; hovever, several sIL1R2 forms ranging from 45 and 60 kDa are reported.
Similarity:
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
SWISS:
P27930
Gene ID:
7850
Database links:Entrez Gene: 7850 Human
Omim: 147811 Human
SwissProt: P27930 Human
Unigene: 25333 Human
IL-1RⅡ属于新的信号受体家族TollSignal transduction通路蛋白,IL-1RⅡ在免疫和炎症反应的研究中占重要地位,是在很多感染 、炎症性致病机制中起决定性作用的cell factor,几乎可作用于全身各种细胞,IL-1 与IL-1RⅡ的结合可导致大量与免疫反应和炎症相关基因的表达 ,包括cell factor、cell factor受体、急性期反应物、生长因子、胞外基质、粘附分子等。IL-1RⅡ/NF-κB信号通路是机体免疫反应中十分重要的组成部分。Product Picture Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human A549 cell lysates
Primary: Anti-1R2 (SL2595R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 68 kDa
Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-1R2) Polyclonal Antibody, Unconjugated (SL2595R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control: RSC96 cells(blue).
Primary Antibody: Rabbit Anti-IL-1R2 antibody(SL2595R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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