Product Name	CD163
Chinese Name	CD163抗体
Alias	Scavenger receptor cysteine-rich type 1 protein M130; CD 163; CD163 antigen; CD163 molecule; Hemoglobin Scavenger Receptor; M130; M130 antigen precursor; Macrophage associated antigen; MM130; C163A_HUMAN.
literatures	Specific References  (72)     |     SL2527R has been referenced in 72 publications. [IF=20.722] Meng Lin. et al. CRISPR-based in situ engineering tumor cells to reprogram macrophages for effective cancer immunotherapy. Nano Today. 2022 Feb;42:101359  IF ;  Mouse.   PubMed:10.1016/j.nantod.2021.101359 [IF=18.187] Peng, Liao. et al. Integrating single-cell RNA sequencing with spatial transcriptomics reveals immune landscape for interstitial cystitis. SIGNAL TRANSDUCT TAR. 2022 May;7(1):1-13  IF ;  Human.   PubMed:35589692 [IF=11.205] Yingchun Luo. et al. Akkermansia muciniphila prevents cold-related atrial fibrillation in rats by modulation of TMAO induced cardiac pyroptosis. EBIOMEDICINE. 2022 Aug;82:104087  IF ;  Rat.   PubMed:35797768 [IF=11.092] Fangyu Qiao. et al. Hybrid Cell Membrane-Functionalized Matrixes for Modulating Inflammatory Microenvironment and Improving Bone Defect Repair. ADV HEALTHC MATER. 2023 Apr;:2203047  IF ;  Rat.   PubMed:37059691 [IF=10.317] Yang G et al. A multifunctional anti-inflammatory drug that can specifically target activated macrophages, massively deplete intracellular H 2 O 2, and produce large amounts CO for a highly efficient treatment of osteoarthritis. Biomaterials . 2020 Oct;255:120155.  ICF ;  Mouse.   PubMed:32554130 [IF=10.317] Yang G et al. A multifunctional anti-inflammatory drug that can specifically target activated macrophages, massively deplete intracellular H2O2, and produce large amounts CO for a highly efficient treatment of osteoarthritis. Biomaterials. 2020 Oct;255:120155.  ICF ;  Mouse.   PubMed:32554130 [IF=9.071] Giorgio Caratti. et al. Macrophagic AMPKα1 orchestrates regenerative inflammation induced by glucocorticoids. EMBO REP. 2022 Dec 15  IF ;  Mouse.   PubMed:36520372 [IF=8.947] Junpeng Chen. et al. An all-in-one CO gas therapy-based hydrogel dressing with sustained insulin release, anti-oxidative stress, antibacterial, and anti-inflammatory capabilities for infected diabetic wounds. ACTA BIOMATER. 2022 Apr;:  WB,IF ;  Mouse.   PubMed:35500813 [IF=8.897] Liu, Houli. et al. Immunomodulatory hybrid bio-nanovesicle for self-promoted photodynamic therapy. Nano Res. 2022 Feb;:1-10  WB ;  Mouse.   PubMed:10.1007/s12274-021-4050-3 [IF=8.579] Tong L et al. ACT001 reduces the expression of PD-L1 by inhibiting the phosphorylation of STAT3 in glioblastoma. Theranostics. 2020 May 1;10(13):5943-5956.  IHC ;  Mouse.   PubMed:32483429 [IF=8.2] Lee, Jin, et al. "Preventive Inhibition of Liver Tumorigenesis by Systemic Activation of Innate Immune Functions." Cell Reports 21.7 (2017): 1870-1882.  IHC-F ;  Mouse.   PubMed:29141219 [IF=8.109] Thiago DeSouza-Vieira. et al. Heme Oxygenase-1 Induction by Blood-Feeding Arthropods Controls Skin Inflammation and Promotes Disease Tolerance. Cell Rep. 2020 Oct;33:108317  FC ;  Mouse.   PubMed:33113362 [IF=7.561] Pengfei Li. et al. Comparative Proteomic Analysis of Polarized Human THP-1 and Mouse RAW264.7 Macrophages. Front Immunol. 2021; 12: 700009  IF ;  Human, Mouse.   PubMed:34267761 [IF=7.21] Oh, Jisu, et al. "Deletion of Macrophage Vitamin D Receptor Promotes Insulin Resistance and Monocyte Cholesterol Transport to Accelerate Atherosclerosis in Mice." Cell Reports (2015).  Mouse.   PubMed:25801026 [IF=6.922] Shanping Wang. et al. Isosteviol Sodium Exerts Anti-Colitic Effects on BALB/c Mice with Dextran Sodium Sulfate-Induced Colitis Through Metabolic Reprogramming and Immune Response Modulation. J Inflamm Res. 2021; 14: 7107–7130  IF ;  Mouse.   PubMed:34992409 [IF=6.63] Yin, Lili. et al. Extracellular vesicles derived from M2-polarized tumor-associated macrophages promote immune escape in ovarian cancer through NEAT1/miR-101-3p/ZEB1/PD-L1 axis. CANCER IMMUNOL IMMUN. 2022 Nov;:1-16  IF ;  Human, Mouse.   PubMed:36319716 [IF=6.543] Wang Shanping. et al. Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-κB Pathway. Oxid Med Cell Longev. 2022;2022:4636618  IF ;  Mouse.   PubMed:35126813 [IF=6.353] Luo Haiyun. et al. Concentrated growth factor regulates the macrophage-mediated immune response. Regen Biomater. 2021 Sep;8(6):  IF ;  mouse.   PubMed:34513006 [IF=6.244] Yang Chenbo. et al. Relationship Between PTEN and Angiogenesis of Esophageal Squamous Cell Carcinoma and the Underlying Mechanism. Front Oncol. 2021 Nov;0:4638  ICC ;  Human.   PubMed:34796109 [IF=6.208] Pei-Wen Wang. et al. Hepatic Stellate Cell Modulates the Immune Microenvironment in the Progression of Hepatocellular Carcinoma. INT J MOL SCI. 2022 Jan;23(18):10777  WB ;  Rat.   PubMed:36142683 [IF=5.984] Nina P. Connolly. et al. Elevated fibroblast growth factor-inducible 14 expression transforms proneural-like gliomas into more aggressive and lethal brain cancer. 2021 May 15  IF ;  Rat.   PubMed:33991013 [IF=5.97] Rashad S et al. Intracellular S1P Levels Dictate Fate of Different Regions of the 4 Hippocampus following Transient Global Cerebral Ischemia.Neuroscience, 384, 188–202.  IHF ;  Rat.   PubMed:10.1016/j.neuroscience.2018.05.015 [IF=5.76] Das, Subhamoy, et al. "Syndesome Therapeutics for Enhancing Diabetic Wound Healing." Advanced Healthcare Materials (2016).  IHC-P ;  Mouse.   PubMed:27385307 [IF=5.714] Shuling Zhang. et al. Methionine enkephalin suppresses lung cancer metastasis by regulating the polarization of tumor-associated macrophages and the distribution of myeloid-derived suppressor cells in the tumor microenvironment and inhibiting epithelial-mesenchymal transition. INT IMMUNOPHARMACOL. 2023 May;118:110064  IF ;  Mouse.   PubMed:36989897 [IF=5.58] Franken, Lars, et al. "Splenic red pulp macrophages are intrinsically superparamagnetic and contaminate magnetic cell isolates." Scientific Reports5 (2015).  Mouse.   PubMed:26260698 [IF=5.554] Okonogi N et al. Combination therapy of intravenously injected microglia and radiotherapy prolongs survival in a rat model of spontaneous malignant glioma.International Journal of Radiation Oncology*Biology*Physics. 2018.  IHC-P ;  Rat.   PubMed:10.1016/j.ijrobp.2018.06.018 [IF=5.51] Kobori et al. Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis. (2018) Front.Immunol. 9:334  FC ;  Mouse.   PubMed:29559970 [IF=5.501] Haneen S. Dwaib. et al. Phosphorus Supplementation Mitigates Perivascular Adipose Inflammation–Induced Cardiovascular Consequences in Early Metabolic Impairment. J Am Heart Assoc. 2021;0:e023227  FC ;  Rat.   PubMed:34873915 [IF=5.29] Protti et al. Bone marrow transplantation modulates tissue macrophage phenotype and enhances cardiac recovery after subsequent acute myocardial infarction. (2016) J.Mol.Cell.Cardiol. 90:120-8  IHC ;  Mouse.   PubMed:26688473 [IF=5.268] An-tian Xu. et al. Effects of strontium-incorporated micro/nano rough titanium surfaces on osseointegration via modulating polarization of macrophages. Colloid Surface B. 2021 Nov;207:111992  IHC ;  Rat.   PubMed:34391168
Research Area	Cardiovascular  immunology  The cell membrane受体
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	130kDa
Detection molecular weight	134(non-reduced)/155 kDa
Cellular localization	The cell membrane Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CD163: 1001-1121/1156 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011] Function: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. Subcellular Location: Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells. Tissue Specificity: Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood. Post-translational modifications: A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs. Phosphorylated. Similarity: Contains 9 SRCR domains. SWISS: Q86VB7 Gene ID: 9332 Database links: Entrez Gene: 9332 Human Omim: 605545 Human SwissProt: Q86VB7 Human Unigene: 504641 Human CD163是SRCR超家族成员之一，又称为血红蛋白清道夫受体（hemoglobin scavenger receptor, HbSR）、M130或p155。CD163的表达水平受很多因素调节，糖皮质激素及抗炎介质如IL-10以及IL-6能上调CD163, 而促炎介质如脂多糖（lipopolysaccharide, LPS）、γ-Interferon（interferon-γ， IFN-γ）以及TNF-α则抑制CD163表达。 CD163是一种I型膜蛋白，又称为M130抗原、Ber-Mac3、Ki-M8或SM4。CD163限制性表达于单核/巨噬细胞系，所有循环系统的单核细胞和大多数组织（除外淋巴滤泡套区和生发中心）的巨噬细胞均阳性表达，主要用于单核/巨噬细胞的检测。
Product Picture	Sample: Lane1: Brain (Rat) Lysate at 30 ug Lane2: Colon carcinoma(Human) Lysate at 30 ug Primary: Anti- CD163 (SL2527R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD163) Polyclonal Antibody, Unconjugated (SL2527R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163/M130 Polyclonal Antibody, unconjugated (SL2527R) at 1:100 for 40 minutes at 37°C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37°C. DAPI nuclear stain employed. Image shows membrane staining of testicular macrophages in the interstitial compartment of the testis, while cells in the seminiferous tubules are negative. Blank control (Black line): U2OS. Primary Antibody (green line): Rabbit Anti-CD163 antibody (SL2527R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% paraformaldehyde for 10 min at room temperature. Cells incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were then stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-CD163 antibody (SL2527R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Cartpieces

• 
• 1

  1Delete

• 
• 5

  5Delete

• 
• S

  SDelete

• 
• R

  RDelete

• 
• +

  +Delete

• 
• 2

  2Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• S

  SDelete

• 
• S

  SDelete

• 

• Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• R

  RDelete

• 
• 5

  5Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout