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Product Name EP4 Chinese Name 前列腺素E受体蛋白4抗体 Alias Prostaglandin E Receptor EP4; EP 4; EP4; EP-4; EP4R; MGC126583; PE2R4_HUMAN; PGE receptor EP4 subtype; PGE2 receptor EP4 subtype; Prostaglandin E receptor 4; Prostaglandin E receptor 4 subtype EP4; Prostaglandin E2 receptor; Prostaglandin E2 receptor EP4 subtype; Prostanoid EP4 receptor; PTGER 2; PTGER 4; PTGER2; PTGER4. Research Area Signal transduction G protein-coupled receptor Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Applications WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=2ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 53kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from mouse EP4 : 1-100/513 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
Function:
Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
Subunit:
Interacts with FEM1A.
Subcellular Location:
Cell membrane.
Tissue Specificity:
High in intestine and in peripheral blood mononuclear cells; low in lung, kidney, thymus, uterus, vasculature and brain. Not found in liver, heart, retina oe skeletal muscle.
Post-translational modifications:
Phosphorylation mediates agonist-mediated desensitization by promoting cytoplasmic retention.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
SWISS:
P32240
Gene ID:
19219
Database links:Entrez Gene: 5734 Human
Entrez Gene: 19219 Mouse
Entrez Gene: 443222 Sheep
Omim: 601586 Human
SwissProt: P35408 Human
SwissProt: P32240 Mouse
Unigene: 199248 Human
Unigene: 18509 Mouse
Unigene: 16062 Rat
Product Picture Sample:
Lane 1: Mouse Uterus tissue lysates
Lane 2: Mouse Small intestine tissue lysates
Lane 3: Mouse Kidney tissue lysates
Lane 4: Mouse Prostate tissue lysates
Primary: Anti-EP4 (SL24918R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 55 kD
Blank control:Ctll-2.
Primary Antibody (green line): Rabbit Anti-EP4 antibody (SL24918R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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