Product Name	TWIST
Chinese Name	TWIST蛋白抗体
Alias	ACS3; B-HLH DNA binding protein; bHLHa38; BPES2; BPES3; Class A basic helix-loop-helix protein 38; CRS1; H-twist; OTTHUMP00000116043; SCS; Twist basic helix loop helix transcription factor 1; Twist homolog 1 (Drosophila); Twist homolog 1; TWIST homolog of drosophila; Twist related protein 1; Twist-related protein 1; Twist1; TWST1_HUMAN.
literatures	Specific References  (14)     |     SL2441R has been referenced in 14 publications. [IF=10.679] Pan J et al. lncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating Wnt/β-catenin signaling. Mol Cancer. 2020 Jan 15;19(1):9.  IHC-P ;  Mouse.   PubMed:31941509 [IF=7.333] Shen et al. Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy. (2018) J.Hematol.Oncol. 11:95  WB ;  Human.   PubMed:30016968 [IF=5.572] Haoming Chen. et al. Nicotine exposure exacerbates silica-induced pulmonary fibrosis via STAT3-BDNF-TrkB-mediated epithelial-mesenchymal transition in alveolar type II cells. FOOD CHEM TOXICOL. 2023 May;175:113694  WB ;  Mouse,Human.   PubMed:36868510 [IF=4.522] Chang J et al. miR‐363‐3p inhibits migration, invasion, and epithelial–mesenchymal transition by targeting NEDD9 and SOX4 in non‐small‐cell lung cancer. J Cell Physiol. 2019 Jul 22.  WB ;  Human.   PubMed:31332786 [IF=4.175] Junmin Li. et al. Circ_ZFR contributes to the paclitaxel resistance and progression of non-small cell lung cancer by upregulating KPNA4 through sponging miR-195-5p. Cancer Cell Int. 2021 Dec;21(1):1-15   PubMed:33407505 [IF=4.12] Wang et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. (2016) Sci.Rep. 6:36543  WB ;  Human.   PubMed:27824118 [IF=3.776] Xiaobei Zhang. et al. The roles and molecular mechanisms of long non-coding RNA WT1-AS in the maintenance and development of gastric cancer stem cells.. HELIYON. 2023 Mar;9(4):e14655-e14655  WB ;  Human.   PubMed:37025896 [IF=3.322] Yonghe Li. et al. SOS1 regulates HCC cell epithelial-mesenchymal transition via the PI3K/AKT/mTOR pathway. BIOCHEM BIOPH RES CO. 2022 Nov;:  WB ;  Human.   PubMed:36403479 [IF=3.25] Xu, Shichen, et al. "Capsaicin inhibits metastasis of human papillary thyroid carcinoma BCPAP cells through modulation of the TRPV1 channel." Food & Function (2017).  Human.   PubMed:29185571 [IF=3.195] Wang Wenjun. et al. LncRNA NEAT1 antagonizes the inhibition of melanoma proliferation, migration, invasion and EMT by Polyphyllin B. N-S ARCH PHARMACOL. 2023 Apr;:1-12  WB ;  Mouse.   PubMed:37004552 [IF=3.19] Zhang, Lei, et al. "High-Throughput RNAi Screening Identifies a Role for the Osteopontin Pathway in Proliferation and Migration of Human Aortic Smooth Muscle Cells." Cardiovascular Drugs and Therapy (2016): 1-15.  IF(ICC) ;  Human.   PubMed:27095116 [IF=3.06] Jin, Meiyuan. et al. Role of ARID1A in the Regulation of Human Trophoblast Migration and Invasion. 2021 Jul 13  WB ;  Human.   PubMed:34255312 [IF=2.952] Jian Shi. et al. FOXP4 promotes laryngeal squamous cell carcinoma progression through directly targeting LEF‑1. Mol Med Rep. 2021 Dec;24(6):1-10  WB ;  human .   PubMed:34590150 [IF=2.058] Ma T et al. MiR-940 inhibits migration and invasion of tongue squamous cell carcinoma via regulatingCXCR2/NF-κB system-mediated epithelial–mesenchymal transition. Naunyn Schmiedebergs Arch Pharmacol. 2019 Jun 18.  WB&IF ;  Human.   PubMed:31214736
Research Area	Tumour  immunology  Neurobiology  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	23kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human TWIST: 71-170/202
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq, Jul 2008]. Function: Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Homodimer or heterodimer with E proteins such as TCF3. ID1 binds preferentially to TCF3 but does not interact efficiently with TWIST1 so ID1 levels control the amount of TCF3 available to dimerize with TWIST1 and thus determine the type of dimer formed. Subcellular Location: Nucleus. Tissue Specificity: Subset of mesodermal cells DISEASE: Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly. Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome. Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. SWISS: Q15672 Gene ID: 7291 Database links: Entrez Gene: 7291 Human Entrez Gene: 22160 Mouse Entrez Gene: 85489 Rat Omim: 601622 Human SwissProt: Q15672 Human SwissProt: P26687 Mouse Unigene: 66744 Human Unigene: 3280 Mouse Unigene: 161904 Rat 转录因子 Twist蛋白是属于碱性螺旋-环-螺旋蛋白家族中的高度保守的转录因子,Twist在抑制Tumour凋亡,促进Tumour细胞的转移发挥一定的作用。
Product Picture	Sample: Siha (human)Cell Lysate at 40 ug Primary: Anti-TWIST protein(SL2441R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 25 kD Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TWIST) Polyclonal Antibody, Unconjugated (SL2441R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TWIST) Polyclonal Antibody, Unconjugated (SL2441R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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