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Product Name B4GALT2 Chinese Name B4GALT2抗体 Alias Beta-1,4-Galactosyltransferase 2; UDP-Galactose:Beta-N-Acetylglucosamine Beta-1,4-Galactosyltransferase 2; UDP-Gal:Beta-GlcNAc Beta-1,4-Galactosyltransferase 2; Beta-1,4-GalTase 2; Beta4Gal-T2; B4Gal-T2; Beta-N-Acetylglucosaminyl-Glycolipid Beta-1,4-Galactosyltransferase 2; UDP-Gal:BetaGlcNAc Beta 1,4- Galactosyltransferase, Polypeptide 2; UDP-Gal:BetaGlcNAc Beta 1,4-Galactosyltransferase, Polypeptide 2; UDP-Gal:BetaGlcNAc Beta 1,4- Galactosyltransferase 2; Beta-4-GalT2; EC 2.4.1.-; B4Gal-T3; B4GT2_HUMAN; Research Area Cell biology Synthesis and Degradation glycoprotein Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 42kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human B4GALT2: 201-300/372 <Cytoplasmic> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene synthesizes N-acetyllactosamine in glycolipids and glycoproteins. Its substrate specificity is affected by alpha-lactalbumin but it is not expressed in lactating mammary tissue. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
Function:
Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose.
Subcellular Location:
Golgi apparatus
Tissue Specificity:
Weakly expressed in various tissues. Highest expression in prostate, testis, ovary, intestine, muscle, and in fetal brain.
Similarity:
Belongs to the glycosyltransferase 7 family.
SWISS:
O60909
Gene ID:
8704
Database links:Entrez Gene: 8704 Human
Entrez Gene: 53418 Mouse
Omim: 604013 Human
SwissProt: O60909 Human
SwissProt: Q9Z2Y2 Mouse
Unigene: 632403 Human
Unigene: 123843 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (Rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B4GALT2) Polyclonal Antibody, Unconjugated (SL23826R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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