Product Name	phospho-Gli1 (T101/ S102/S104)
Chinese Name	磷酸化脑胶质瘤相关蛋白抗体
Alias	Phospho-Gli1 (Thr101/ Ser102/ Ser104); P-Gli1 (Thr101/ Ser102/ Ser104); Gli1 (Phospho-Thr101/ Ser102/ Ser104); Gli 1; Gli1; Gli-1; GLI; GLI Kruppel family member 1; Glioma associated oncogene; Glioma associated oncogene homolog 1 (zinc finger protein); Oncogene GLI; Zfp 5; Zfp5; Zinc finger protein GLI 1; Zinc finger protein GLI1; GLI1_HUMAN.
Product Type	Phosphorylated anti
Research Area	Tumour  Cell biology  Neurobiology  Signal transduction  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Horse, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	118kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/1ml
immunogen	KLH conjugated Synthesised phosphopeptide peptide derived from human Gli1 around the phosphorylation site of T101/S102/S104: IR(p-T)(p-S)P(p-S)SL
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] Function: Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. Subcellular Location: Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1. Tissue Specificity: Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. SWISS: P08151 Gene ID: 2735 Database links: Entrez Gene: 2735 Human Entrez Gene: 14632 Mouse Entrez Gene: 140589 Rat Entrez Gene: 517588 Cow Omim: 165220 Human SwissProt: P08151 Human SwissProt: P47806 Mouse Unigene: 632702 Human Unigene: 391450 Mouse Unigene: 219157 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Gli1 (T101/S102/S104)) Polyclonal Antibody, Unconjugated (SL23798R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Gli1 (T101/S102/S104)) Polyclonal Antibody, Unconjugated (SL23798R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-Gli1 (T101/S102/S104)) Polyclonal Antibody, Unconjugated (SL23798R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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