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Product Name DcR3 Chinese Name 诱捕受体3抗体 Alias DcR3; Decoy Receptor 3; Decoy receptor for Fas ligand; DJ583P15.1.1; M68; OTTHUMP00000031583; TNF6B_HUMAN; TNFRSF6B; TR6; Tumor necrosis factor receptor superfamily member 6B; M68; Tumor necrosis factor receptor superfamily member 6b decoy; UNQ186/PRO212. Research Area Cell biology immunology Apoptosis Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Dog, Cow, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 30kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human DcR3: 51-150/300 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. Several novel members in the TNFR family including DR3, DR4, DR5, and DR6 were recently discovered and function as cell death receptors. Two decoy receptors, DcR1 and DcR2, were recently identified to compete with DR4 and DR5 for their ligand TRAIL binding. A novel decoy receptor was more recently discovered and designated DcR3 and TR6, respectively. Unlike DcR1and DcR2, DcR3 is a soluble rather than a membraneassociated molecule. DcR3 binds to FasL and LIGHT andinhibits FasL and LIGHT induced apoptosis. DcR3 transcript is expressed in a number of lung and coloncarcinomas and in some normal tissues.
Function:
Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis.
Subcellular Location:
Secreted.
Tissue Specificity:
Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.
Similarity:
Contains 4 TNFR-Cys repeats.
SWISS:
O95407
Gene ID:
8771
Database links:Entrez Gene: 8771 Human
Omim: 603361 Human
SwissProt: O95407 Human
Unigene: 434878 Human
Product Picture Sample:
DU145(Human) Cell Lysate at 30 ug
Primary: Anti-DcR3 (SL23737R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 37 kD
Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti- DcR3 (SL23737R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
Sample:
Lymph node (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti- DcR3 (SL23737R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-DcR3 (SL23737R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 32 kD
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DcR3) Polyclonal Antibody, Unconjugated (SL23737R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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