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Product Name Lamin B (Nuclear Loading Control) Chinese Name 核纤层蛋白B(核内参)抗体 Alias lamin B1; LMB1; LMN; LMN2; LMNB 1; LMNB; LMNB1; MGC111419; LMNB1_HUMAN; Lamin-B1. literatures Specific References (3) | SL23709R has been referenced in 3 publications.[IF=14.66] Fischer, Anika, et al. "Differential effects of α4β7 and GPR15 on homing of effector and regulatory T cells from patients with UC to the inflamed gut in vivo." Gut (2015): gutjnl-2015. IHC-F ; Human.[IF=3.361] Yang X et al. HJ22, a Novel derivative of piperine, Attenuates ibotenic acid-induced cognitive impairment, oxidativestress, apoptosis and inflammation via inhibiting the protein-protein interaction of Keap1-Nrf2. Int Immunopharmacol. 2020 Mar 16;83:106383. IHC,WB ; rat.[IF=1.203] Sun X et al. Panaxynol from Saposhnikovia diviaricata exhibits a hepatoprotective effect against lipopolysaccharide + D-Gal N induced acute liver injury by inhibiting Nf-κB/IκB-α and activating Nrf2/HO-1 signaling pathways. Biotech Histochem . 2020 Apr 16;1-9. WB ; mouse.Product Type Internal reference anti Research Area Cell biology Chromatin and nuclear signals Signal transduction Apoptosis Cell type markers Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 64kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Lamin B: 171-270/586 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010].
Function:
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Subunit:
Homodimer. Interacts with lamin-associated polypeptides IA, IB and 2.
Subcellular Location:
Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.
Post-translational modifications:
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
DISEASE:
Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P20700
Gene ID:
4001
Database links:Entrez Gene: 396223 Chicken
Entrez Gene: 4001 Human
Entrez Gene: 16906 Mouse
Omim: 150340 Human
SwissProt: P14731 Chicken
SwissProt: P20700 Human
SwissProt: P14733 Mouse
Unigene: 89497 Human
Unigene: 4105 Mouse
Unigene: 11362 Rat
核膜Maker(Nuclear Envelope Marker)
核纤层蛋白(Lamin) 是紧贴核内膜的一层厚度为20~50nm的纤维蛋白层或纤维网络。核纤层与细胞质骨架、核骨架连成一个整体,一般认为核纤层将核被膜和染色质提供了结构支架。有学者研究认为:lamin蛋白与Apoptosis及衰老有关联,它包括:核纤层蛋白A、核纤层蛋白B、核纤层蛋白C几个不同亚型的蛋白。Product Picture Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-Lamin B (SL23709R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 64 kD
Observed band size: 64 kD
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