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Product Name LTA4H Chinese Name 白三烯A4水解酶抗体 Alias Leukotriene A4 hydrolase LTA4; LTA4 hydrolase; LTA4H; LKHA4_HUMAN. Research Area Tumour Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Pig, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 69kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human LTA4H: 1-100/611 <Cytoplasmic> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Leukotrienes are a group of bioactive compounds which play important roles in immediate hyposensitivity reactions and inflammation. Leukotriene A4 hydrolase hydrolyzes an epoxide moiety of leukotriene A4 (LTA4) to form leukotriene B4 (LTB4). The enzyme also has some peptidase activity.
Function:
Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity.
Subunit:
Monomer.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Isoform 1 and isoform 2 are expressed in monocytes, lymphocytes, neutrophils, reticulocytes, platelets and fibroblasts.
Post-translational modifications:
Phosphorylation at Ser-416 inhibits enzymatic activity.
Similarity:
Belongs to the peptidase M1 family.
SWISS:
P09960
Gene ID:
4048
Database links:Entrez Gene: 4048 Human
Omim: 151570 Human
SwissProt: P09960 Human
Unigene: 524648 Human
Unigene: 104990 Rat
Product Picture Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti- LTA4H (SL23674R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kD
Observed band size: 69 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LTA4H) Polyclonal Antibody, Unconjugated (SL23674R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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