Product Name	LTA4H
Chinese Name	白三烯A4水解酶抗体
Alias	Leukotriene A4 hydrolase LTA4; LTA4 hydrolase; LTA4H; LKHA4_HUMAN.
Research Area	Tumour  Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Dog, Pig, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	69kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human LTA4H: 1-100/611 <Cytoplasmic>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Leukotrienes are a group of bioactive compounds which play important roles in immediate hyposensitivity reactions and inflammation. Leukotriene A4 hydrolase hydrolyzes an epoxide moiety of leukotriene A4 (LTA4) to form leukotriene B4 (LTB4). The enzyme also has some peptidase activity. Function: Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity. Subunit: Monomer. Subcellular Location: Cytoplasm. Tissue Specificity: Isoform 1 and isoform 2 are expressed in monocytes, lymphocytes, neutrophils, reticulocytes, platelets and fibroblasts. Post-translational modifications: Phosphorylation at Ser-416 inhibits enzymatic activity. Similarity: Belongs to the peptidase M1 family. SWISS: P09960 Gene ID: 4048 Database links: Entrez Gene: 4048 Human Entrez Gene: 299732 Rat Omim: 151570 Human SwissProt: P09960 Human SwissProt: P30349 Rat Unigene: 524648 Human Unigene: 104990 Rat
Product Picture	Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- LTA4H (SL23674R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 69 kD Observed band size: 69 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LTA4H) Polyclonal Antibody, Unconjugated (SL23674R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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