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Product Name PIAS4/PIASy Chinese Name 激活STAT蛋白抑制因子4抗体 Alias E3 SUMO protein ligase PIAS4; E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; PAIASgamma; PIAS 4; PIAS gamma; PIAS-gamma; PIAS4; PIAS4_HUMAN; PIASG; PIASgamma; PIASy; Protein inhibitor of activated STAT 4; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; Protein inhibitor of activated STAT protein PIASy; Zinc finger MIZ type containing 6; ZMIZ 6; ZMIZ6. Research Area Cell biology Apoptosis Growth factors and hormones transcriptional regulatory factor Epigenetics Ubiquitin Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Chicken, Pig, Cow, Horse, Zebrafish, ) Applications WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 55kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PIAS4/PIASy : 181-280/510 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The IL-6-type family of cytokines, which includes IL-6 and a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the STAT family are involved in IL-6 family-mediated signal transduction pathways, and upon activation undergo phosphorylation, dimerization and translocation to the nucleus. The duration and intensity of a cell's response to cytokines can be adjusted by the effect of several regulatory mechanisms. One example involves the protein inhibitor of activated signal transducer and activator of transcription (STAT) family (PIAS family) of proteins, which act as negative regulators of STATs in cytokine signaling. PIAS proteins are able to coactivate steroid receptor-dependent transcription as well. Human PIASy is a 510 amino acid transcriptional corepressor of the androgen receptor (AR). In addition, PIASy may regulate p53-mediated events and may direct p53 into a transactivation-independent mode of apoptosis.
Function:
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations.
Subcellular Location:
Nucleus > PML body. Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.
Tissue Specificity:
Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.
Post-translational modifications:
Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activitation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.
Similarity:
Belongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger.
SWISS:
Q8N2W9
Gene ID:
51588
Database links:Entrez Gene: 51588 Human
Entrez Gene: 59004 Mouse
Omim: 605989 Human
SwissProt: Q8N2W9 Human
SwissProt: Q3UGQ2 Mouse
SwissProt: Q9JM05 Mouse
Unigene: 105779 Human
Unigene: 34428 Mouse
Unigene: 12810 Rat
Product Picture Sample:
Testis (Rat) Lysate at 40 ug
Primary: Anti-PIASy (SL23480R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
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