Product Name	ACE2
Chinese Name	血管紧张素转换酶2抗体
Alias	ACE-2; ACE 2; Angiotensin converting enzyme 2; ACE related carboxypeptidase; ACEH; Angiotensin converting enzyme homolog; Angiotensin converting enzyme like protein; Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2; Angiotensin I converting enzyme 2; DKFZP434A014; EC 3.4.17; angiotensin-converting enzyme 2 precursor; ACE2_HUMAN; Angiotensin-converting enzyme 2; ACE-related carboxypeptidase; Angiotensin-converting enzyme homolog; Metalloprotease MPROT15; Processed angiotensin-converting enzyme 2.
literatures	Specific References  (1)     |     SL23444R has been referenced in 1 publications. [IF=3.829] Zhang Xiangjun. et al. DAD3 targets ACE2 to inhibit the MAPK and NF-κB signalling pathways and protect against LPS-induced inflammation in bovine mammary epithelial cells. VET RES. 2022 Dec;53(1):1-13  IF, WB ;  Bovine.   PubMed:36482404
Research Area	Cell biology  immunology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	87kDa
Cellular localization	The cell membrane Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ACE2: 351-450/805 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]. Function: Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. Subunit: Interacts with ITGB1. Interacts with SARS-CoV and HCoV-NL63 spike glycoprotein. Subcellular Location: Processed angiotensin-converting enzyme 2: Secreted. Cell membrane; Single-pass type I membrane protein. Tissue Specificity: Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system. Post-translational modifications: N-glycosylation on Asn-90 may limit SARS infectivity. Proteolytic cleavage by ADAM17 generates a secreted form. Belongs to the peptidase M2 family. Similarity: Belongs to the peptidase M2 family. SWISS: Q9BYF1 Gene ID: 59272 Database links: Entrez Gene: 59272 Human Entrez Gene: 70008 Mouse Entrez Gene: 302668 Rat Omim: 300335 Human SwissProt: Q9BYF1 Human SwissProt: Q8R0I0 Mouse SwissProt: Q5EGZ1 Rat Unigene: 178098 Human Unigene: 13451 Mouse Unigene: 129779 Rat Synthesis and Degradation（Synthesis and Degradation） ACE-2的分布范围比较局限，ACE2主要在心脏、肾脏、睾丸中表达显著， 近来人们发现ACE2也分布在胃肠道、脑和肺脏中。 有关学者在研究心脏和肾脏中发现，ACE2在心肌缺血、肾功能衰竭、动脉粥样硬化和Diabetes并发症中，ACE2对血管紧张素产生和降解过程中有一定的生理作用。 特别是对ACE2在具有生理活性的-活性肽产生过程中的作用更值得进一步研究。 ACE2的发现为Cardiovascular病和肾脏病研究开辟了新天地，并提供了新的治疗靶点，可能导致未来Cardiovascular疾病治疗策略的改变。
Product Picture	Sample: Lane 1: ACE2 knockout (KO) A549 Cell Lysate Lane 2: Human A549 Cell (Control) Lysate Primary: Anti-ACE2 (SL23444R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 87 kD Observed band size: 120 kD Sample: Lane 1: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 2: HepG2 (Human) Cell Lysate at 30 ug Lane 3: 293T (Human) Cell Lysate at 30 ug Primary: Anti-ACE2 (SL23444R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120 kD Observed band size: 120 kD Sample: Lane 1: Testis(Mouse) Lysate at 40 ug Lane 2: Ovary (Mouse) Lysate at 40 ug Lane 3: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 4: HepG2 (Human) Cell Lysate at 30 ug Lane 5: A431 (Human) Cell Lysate at 30 ug Primary: Anti-ACE2 (SL23444R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120 kD Observed band size: 120 kD Sample: Lane 1: ACE2 Protein Overexpression E.coli Lysate(41410P) at 2ug Lane 2: Control group E.coli Lysate(41410P) Primary: Anti-ACE2 (SL23444R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 70 kD A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ACE2) polyclonal Antibody, Unconjugated (SL23444R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-ACE2 antibody (SL23444R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 10,000 events was performed.

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