Product Name	GCLC
Chinese Name	γ谷氨酰半胱氨酸合成酶抗体
Alias	Gamma ECS; Gamma GCS; Gamma glutamylcysteine synthetase; Gamma-ECS; Gamma-glutamylcysteine synthetase; GCL; GCLC; GCS; GCS Heavy Chain antibody GLCL; GLCLC; GLCLR; Glutamate cysteine ligase catalytic subunit; Glutamate cysteine ligase (gamma glutamylcysteine synthetase) regulatory (30.8kD); Glutamate cysteine ligase catalytic subunit; Glutamate cysteine ligase modifier subunit; Glutamate cysteine ligase regulatory protein; Glutamate cysteine ligase regulatory subunit; Glutamate--cysteine ligase catalytic subunit; GSH1_HUMAN.
literatures	Specific References  (5)     |     SL23393R has been referenced in 5 publications. [IF=8.355] Shi S et al. Homologous-targeting biomimetic nanoparticles for photothermal therapy and Nrf2-siRNA amplified photodynamic therapy against oral tongue squamous cell carcinoma. Chemical Engineering Journal, 2020, 124268.  WB ;  Human.   PubMed:doi:10.1016/j.cej.2020.124268 [IF=4.253] Mengru Li. et al. The leaves of Scutellaria baicalensis Georgi attenuate brain aging in D-galactose-induced rats via regulating glutamate metabolism and Nrf2 signaling pathway. EXP GERONTOL. 2022 Dec;170:111978  WB ;  Rat.   PubMed:36244586 [IF=4.014] Zhang Kaixin. et al. MiR-129-3p regulates ferroptosis in the liver of Selenium-deficient broilers by targeting SLC7A11. POULTRY SCIENCE. 2022 Oct;:102271  WB ;  Chicken.   PubMed:10.1016/j.psj.2022.102271 [IF=3.909] Li ZP et al. Protective roles of Amanita caesarea polysaccharides against Alzheimer's disease via Nrf2 pathway. (2018) Int J Biol Macromol.121:29-37.  WB ;  Mouse.   PubMed:30290256 [IF=2.767] Dezhuan Da. et al. Glutamate-cysteine ligase catalytic and its modifier function as novel immunotargets in gastric adenocarcinoma. Asian J Surg. 2022 Mar;:  IHC ;  Other.   PubMed:35241341
Research Area	Tumour  Cell biology  immunology  Neurobiology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, Zebrafish, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	73kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GCLC: 351-450/637
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit), also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC). g-GCSc is expressed in hemocytes, brain, liver and kidney. g-GCSc associates with a regulatory or modifier subunit, g-GCSm (g-glutamylcysteine synthetase light subunit), to form a heterodimer, g-GCS. g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of g-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of g-GCS. Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, which may protect against chemically induced carcinogenesis. A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels. Defects in GCLC are the cause of hemolytic anemia. Subunit: Heterodimer of a catalytic heavy chain and a regulatory light chain. DISEASE: Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450]. Similarity: Belongs to the glutamate--cysteine ligase type 3 family. SWISS: P48506 Gene ID: 2729 Database links: Entrez Gene: 2729 Human Entrez Gene: 14629 Mouse Entrez Gene: 25283 Rat Omim: 606857 Human SwissProt: P48506 Human SwissProt: P97494 Mouse SwissProt: P19468 Rat Unigene: 654465 Human Unigene: 89888 Mouse Unigene: 8365 Rat
Product Picture	Sample: Kidney (Mouse) Lysate at 40 ug Kidney (Rat) Lysate at 40 ug Primary: Anti-GCLC (SL23393R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 73 kD Observed band size: 73 kD Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Rat Liver tissue lysates Lane 3: Human HepG2 cell lysates Primary: Anti-GCLC (SL23393R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 73 kDa Observed band size: 73 kDa Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCLC) Polyclonal Antibody, Unconjugated (SL23393R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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