Product Name	E2F1
Chinese Name	转录因子E2F-1抗体
Alias	E2F 1; E2F transcription factor 1; E2F-1; E2f1 E2F transcription factor 1; KIAA4009; mKIAA4009; OTTHUMP00000030661; PBR 3; PBR3; PRB binding protein E2F 1; PRB-binding protein E2F-1; RBAP 1; RBAP-1; RBAP1; RBBP 3; RBBP-3; RBBP3; RBP 3; RBP3; Retinoblastoma associated protein 1; Retinoblastoma binding protein 3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1; E2F1_HUMAN.
literatures	Specific References  (2)     |     SL23184R has been referenced in 2 publications. [IF=5.652] Haijun Sun. et al. WD Repeat Domain 43 promotes malignant progression of non-small cell lung cancer by regulating CDK2. INT J BIOCHEM CELL B. 2022 Aug;:106293  WB ;  Human.   PubMed:10.1016/j.biocel.2022.106293 [IF=4.315] Xiaoling Xu. et al. ATPR regulates human mantle cell lymphoma cells differentiation via SOX11/CyclinD1/Rb/E2F1. Cell Signal. 2022 Feb;:110280  WB ;  Human.   PubMed:35151831
Research Area	Tumour  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Rabbit, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	46kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human E2F1: 221-320/437
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008] Function: Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. Subunit: Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1. Interacts with ARID3A. Binds EAPP. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. Similarity: Belongs to the E2F/DP family. SWISS: Q01094 Gene ID: 1869 Database links: Entrez Gene: 1869 Human Entrez Gene: 13555 Mouse Entrez Gene: 399489 Rat Omim: 189971 Human SwissProt: Q01094 Human SwissProt: Q61501 Mouse SwissProt: O09139 Rat Unigene: 654393 Human Unigene: 18036 Mouse Unigene: 72471 Rat transcriptional regulatory factor（Transcriptin Regulators） E2F1—属于调节性转录因子E2F家族。有学者认为：E2F-1既可作为癌基因起作用，又可作为抑癌基因起作用。其不同可能由细胞中其他生长促进或抑制性蛋白质水平和（或）活性决定，同时与细胞所处环境及器官特异性有关。在控制细胞周期和Tumour抑制基因蛋白的活性方面起关键作用。
Product Picture	Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug Lane 2: A673 (Human) Cell Lysate at 30 ug Lane 3: Molt-4 (Human) Cell Lysate at 30 ug Lane 4: U251 (Human) Cell Lysate at 30 ug Primary: Anti-E2F1 (SL23184R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55-60 kD Observed band size: 58 kD

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