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Product Name GSTP1 Chinese Name 谷胱甘肽硫转移酶pi抗体 Alias Glutathione S-transferase P; Glutathione S-Transferase pi; GST-Pi; DFN7; Gst-P1; FAEES3; Fatty Acid Ethyl Ester Synthase III; Glutathione S Transferase Pi; GST3; GSTP1; GST pi; PI; GSTP1_MOUSE. Research Area Tumour Cell biology immunology Neurobiology transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Mouse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 23kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from mouse GSTP1: 81-180/210 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Glutathione S-transferases (GSTs) function in the metabolic detoxification of various environmental carcinogens and lipid hydroperoxides. Members of the murine GSTP (glutathione S-transferase pi) family, termed Gstp1 and Gstp2, are linked to drug resistance and are markers for many cancers. Gstp proteins modulate cell signaling by interacting with c-Jun N-terminal kinase (JNK), and may play a protective role in the development of spontaneous tumors. Gstp has been found in substantia nigra and may be associated with reactive oxygen species-induced neurological disorders such as Parkinson’s disease and may additionally protect against endothelial dysfunction induced by tobacco smoke exposure.
Function:
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Subunit:
Homodimer.
Tissue Specificity:
Ubiquitously expressed.
Similarity:
Belongs to the GST superfamily. Pi family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain.
SWISS:
P09211
Gene ID:
14870
Database links:Entrez Gene: 2950 Human
Entrez Gene: 14870 Mouse
Omim: 134660 Human
SwissProt: P09211 Human
SwissProt: P19157 Mouse
Unigene: 523836 Human
Unigene: 87063 Rat
GST-Pi是GST家族中的一种主要的同工酶,广泛存在于全身器官中,尤以肝肾含量高,具有解毒功能。在多种Tumour中高表达。目前认为是Tumour细胞产生耐药的一种标记,与Tumour的耐药(阿霉素、顺铂、氮芥、环磷酰胺和瘤可宁等)有关。该抗体主要用于细胞耐药方面的研究。Product Picture Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GSTP1) Polyclonal Antibody, Unconjugated (SL23151R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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