Product Name	Ki-67
Chinese Name	Ki67蛋白抗体
Alias	Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_HUMAN.
literatures	Specific References  (4)     |     SL23105R has been referenced in 4 publications. [IF=5.572] Ipek Ozturk. et al. Therapeutic role of melatonin on acrylamide-induced hepatotoxicity in pinealectomized rats: Effects on oxidative stress, NF-κB signaling pathway, and hepatocellular proliferation. FOOD CHEM TOXICOL. 2023 Apr;174:113658  IHC ;  Rat.   PubMed:36780936 [IF=5.116] Mengna Duan. et al. Epidermal stem cell-derived exosomes promote skin regeneration by downregulating transforming growth factor-β1 in wound healing. Stem Cell Res Ther. 2020 Dec;11(1):1-11  IHC ;  Rat.   PubMed:33097078 [IF=3.202] Luo J et al. The effects of aberrant expression of LncRNA DGCR5/miR-873-5p/TUSC3 in lung cancer cell progression.Cancer Med. 2018 May 23.  IHC ;  Mouse.   PubMed:29790668 [IF=1.664] Song N et al. Synergistic anti-tumor effects of dasatinib and dendritic cell vaccine onmetastatic breast cancer in a mouse model. Oncology Letters.2018.  IHC-P ;  Mouse.   PubMed:10.3892/ol.2018.8188
Research Area	Tumour  Cell biology  Neurobiology  Cyclin  Cell differentiation  Cell type markers  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Horse, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	358kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Ki-67: 601-700/3256
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation. Function: Thought to be required for maintaining cell proliferation. Subcellular Location: Nucleus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes. Similarity: Contains 1 FHA domain. SWISS: P46013 Gene ID: 4288 Database links: Entrez Gene: 4288 Human Entrez Gene: 17345 Mouse Entrez Gene: 246042 Rat Omim: 176741 Human SwissProt: P46013 Human SwissProt: Q91VE6 Mouse SwissProt: Q5RJM0 Rat Unigene: 689823 Human Unigene: 80976 Human Unigene: 4078 Mouse Unigene: 233802 Rat 细胞增殖Maker(Proliferation Marker) Ki67与PCNA一样，为细胞增殖的一种标记，在Apoptosis中S、G2 、M期均有表达，G0期缺如。 Ki-67增殖指数高低与许多Tumour的分化程度、浸润、转移以及预后密切相关，因此被广泛作为各种恶性Tumour的必检项目之一。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (SL23105R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control (blue line): Hela (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-Ki-67 antibody (SL23105R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test. Blank control:HL-60. Primary Antibody (green line): Rabbit Anti-Ki-67 antibody (SL23105R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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