Product Name	FOXP3
Chinese Name	叉头蛋白P3抗体
Alias	Forkhead box protein P3; forkhead box P3; Scurfin; Forkhead box protein P3, C-terminally processed; Forkhead box protein P3 41 kDa form; JM2; AIID; IPEX; PIDX; XPID; DIETER; FOXP3_HUMAN;
literatures	Specific References  (1)     |     SL23074R has been referenced in 1 publications. [IF=8.44] Jia, Yu. et al. The treatment of Qibai Pingfei Capsule on chronic obstructive pulmonary disease may be mediated by Th17/Treg balance and gut-lung axis microbiota. J TRANSL MED. 2022 Dec;20(1):1-21  WB, IHC ;  Rat.   PubMed:35729584
Research Area	Cell biology  immunology  transcriptional regulatory factor  lymphocyte
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1ug/Test ICC=1:100  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	47kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human FoxP3: 351-431/431
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]. Function: Probable transcription factor. Plays a critical role in the control of immune response. Subunit: Interacts with IKZF3. Subcellular Location: Nucleus (Potential). Post-translational modifications: Acetylation on lysine residues stabilizes FOXP3 and promotes differentiation of T-cells into induced regulatory T-cells (iTregs) associated with suppressive functions. Deacetylated by SIRT1. DISEASE: Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy. Similarity: Contains 1 C2H2-type zinc finger. Contains 1 fork-head DNA-binding domain. SWISS: Q9BZS1 Gene ID: 50943 Database links: Entrez Gene: 50943 Human Entrez Gene: 20371 Mouse Entrez Gene: 317382 Rat Omim: 300292 Human SwissProt: Q9BZS1 Human SwissProt: Q99JB6 Mouse SwissProt: D3ZKI1 Rat Unigene: 247700 Human Unigene: 182291 Mouse
Product Picture	Sample: Lane 1: Thymus (Mouse) Lysate at 40 ug Lane 2: Spleen (Mouse) Lysate at 40 ug Lane 3: Lymph node (Mouse) Lysate at 40 ug Lane 4: Small intestine (Mouse) Lysate at 40 ug Lane 5: Thymus (Rat) Lysate at 40 ug Lane 6: Spleen (Rat) Lysate at 40 ug Lane 7: Lymph node (Rat) Lysate at 40 ug Lane 8: Small intestine (Rat) Lysate at 40 ug Primary: Anti-FoxP3 (SL23074R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43/45 kD Observed band size: 45 kD U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (FoxP3) polyclonal Antibody, Unconjugated (SL23074R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-FoxP3 antibody (SL23074R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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