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Product Name HES5 Chinese Name 发状分裂相关增强子-5抗体 Alias BHLHB38; Transcription factor HES-5; Class B basic helix-loop-helix protein 38 (bHLHb38); Hairy and enhancer of split 5; HES 5; HES5 protein; MGI:104876; HES5_HUMAN. Research Area Signal transduction Stem cells transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Cow, Horse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 18kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human HES5: 31-130/166 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer. [provided by RefSeq, Dec 2008]
Function:
Transcriptional repressor of genes that require a bHLH protein for their transcription
Subunit:
Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.
Subcellular Location:
Nucleus.
Tissue Specificity:
Expressed in fetal heart and brain tumors.
Similarity:
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
SWISS:
Q5TA89
Gene ID:
388585
Database links:Entrez Gene: 388585 Human
Entrez Gene: 15208 Mouse
SwissProt: Q5TA89 Human
SwissProt: P70120 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (Human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Hes5) Polyclonal Antibody, Unconjugated (SL23071R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Hes5) Polyclonal Antibody, Unconjugated (SL23071R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Hes5) polyclonal Antibody, Unconjugated (SL23071R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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